CD33L3 (Active) protein (Biotin)

N° du produit ABIN7319803

Cette protéine Recombinant CD33L3 est exprimée dans Human Cells.

Aperçu rapide pour CD33L3 (Active) protein (Biotin) (ABIN7319803)

Antigène: CD33L3

Type de proteíne: Recombinant

Activité biologique: Active

Origine: Humain

Source: Human Cells

Pureté: >90 % as determined by reducing SDS-PAGE.

Détail du produit

Purification/Conjugué: Biotin

Fonction: Recombinant Human Sialic acid-binding Ig-like lectin 15/Siglec-15/CD33L3 (C-FC-Avi) Biotinylated

Séquence: Phe20-Thr263

Attributs du produit: Biotinylated Recombinant Human Sialic Acid-binding Ig-like Lectin 15 is produced by our Mammalian expression system and the target gene encoding Phe20-Thr263 is expressed with a Fc, Avi tag at the C-terminus.

niveau d'endotoxine: < 1.0 EU per μg as determined by the LAL method.

Biological Activity Comment: Immobilized Human Siglec-15-Fc-Avi (Cat#CY14) at 2μg/ml (100 μl/well) can bind Anti-Human Siglec15 mAb. The ED50 of Anti-Human Siglec15 mAb is 13.7 ng/ml.

Information d'application

Commentaires:

58-75 kDa

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: Please refer to the printed manual for detailed information.

Buffer: Lyophilized from a 0.2 μm filtered solution of PBS, 150 mM NaCl, 0.3 % Chaps, 5 % Trehalose, pH 7.4.

Stock: 4 °C,-20 °C,-80 °C

Stockage commentaire: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Détail du antigène

Antigène: CD33L3

Sujet:

Background: Human Siglec-15 is a transmembrane glycoprotein in the Siglec family. Siglecs are type I transmembrane proteins where the NH3+-terminus is in the extracellular space and the COO--terminus is cytosolic. Each Siglec contains an N-terminal V-type immunoglobulin domain (Ig domain) which acts as the binding receptor for sialic acid. These lectins are placed into the group of I-type lectins because the lectin domain is an immunoglobulin fold. All Siglecs are extended from the cell surface by C2-type Ig domains which have no binding activity. Siglecs differ in the number of these C2-type domains. Human Siglec-15 consists of a 244 amino acid (aa) extracellular domain (ECD) with two Ig-like domains, a 21 aa transmembrane segment, and a 44 aa cytoplasmic domain. Siglec-15 function is important for osteoclast formation and TRANCE/RANK Ligand signaling in osteoclasts.

Synonym: Sialic acid-binding Ig-like lectin 15, Siglec-15, CD33 antigen-like 3, CD33L3

Poids moléculaire: 55.3 kDa

UniProt: Q6ZMC9