AMPK (A2/B2/G1) (Active) Protéine

N° du produit ABIN7317040

Cette protéine Recombinant AMPK (A2/B2/G1) est exprimée dans Baculovirus infected Insect Cells.

Aperçu rapide pour AMPK (A2/B2/G1) (Active) Protéine (ABIN7317040)

Antigène: AMPK (A2/B2/G1)

Type de proteíne: Recombinant

Activité biologique: Active

Origine: Humain

Source: Baculovirus infected Insect Cells

Pureté: > 90 % as determined by reducing SDS-PAGE.

Détail du produit

Fonction: Recombinant Human AMPK (G1/B2/A2) Heterotrimer Protein (Active)

Séquence: Met 1-Pro 331

Attributs du produit: A DNA sequence encoding the human PRKAG1 (P54619) (Met 1-Pro 331), constructed the plasmid 1, A DNA sequence encoding the human PRKAB2 (O43741) (Met 1-Ile 272), constructed the plasmid 2, A DNA sequence encoding the human PRKAA2 (P54646) (Met 1-Arg 552) was fused with the N-terminal polyhistidine-tagged GST tag at the N-terminus, constructed the plasmid 3. The three plasmids were co-expressed and the heterotrimer was purified.

niveau d'endotoxine: < 1.0 EU per μg of the protein as determined by the LAL method.

Biological Activity Comment: The specific activity was determined to be 6 nmol/min/mg using synthetic SAMS peptide (HMRSAMSGLHLVKRR) as substrate.

Information d'application

Commentaires:

35, 37 & 95 kDa

Restrictions: For Research Use only

Stockage

Format: Frozen, Liquid

Buffer: Supplied as sterile 50 mM Tris, 200 mM NaCl, 1 mM EDTA, 1 mM DTT, 0.5 mM PMSF, 10 % gly, 1 mM GSH, pH 7.4

Stock: -20 °C

Stockage commentaire: Store at < -20°C, stable for 6 months. Please minimize freeze-thaw cycles.

Détail du antigène

Antigène: AMPK (A2/B2/G1)

Sujet: AMPK,AMPKa1

Poids moléculaire: 158 (38+30+90) kDa

UniProt: P54619