ICOSLG Protein (AA 19-258) (His-Avi Tag)

N° du produit ABIN7273967

Protéine Recombinant ICOSLG exprimée dans HEK-293 Cells.

Aperçu rapide pour ICOSLG Protein (AA 19-258) (His-Avi Tag) (ABIN7273967)

Antigène: ICOSLG (Inducible T-Cell Co-Stimulator Ligand (ICOSLG))

Type de proteíne: Recombinant

Activité biologique: Active

Origine: Humain

Source: HEK-293 Cells

Pureté: > 95 % as determined by Bis-Tris PAGE,> 90 % as determined by HPLC

Détail du produit

Attributs du protein: AA 19-258

Purification/Conjugué: Cette ICOSLG protéine est marqué à la His-Avi Tag.

Fonction: Non-biotinylated Human B7-H2/ICOSLG Protein

Séquence: Asp19-Ser258

Attributs du produit: Recombinant Human B7-H2/ICOSLG Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains Asp19-Ser258.

Stérilité: 0.22 μm filtered

niveau d'endotoxine: Less than 1 EU per μg by the LAL method.

Biological Activity Comment: Human ICOS, hFc Tag (Q50A) captured on CM5 Chip via Protein A can bind Human B7-H2, His Tag with an affinity constant of 41.98 nM as determined in SPR assay (Biacore T200).,Immobilized Human B7-H2, His Tag at 1 ug/ml (100 ul/Well) on the plate. Dose response curve for Human ICOS, hFc Tag with the EC50 of 15.2 ng/ml determined by ELISA (QC Test).,Human B7-H2 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.,The purity of Human B7-H2 is greater than 90% as determined by SEC-HPLC.

Information d'application

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 μg or less, at 1 mg/mL for 500 μg or 1 mg lyophilized powder. Do not mix by vortex or vigorous shaking.

Buffer: Lyophilized from 0.22 μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.

Stock: -20 °C,-80 °C

Stockage commentaire: -20 to -80°C for 12 months as supplied from date of receipt.,-80°C for 3 months after reconstitution.,Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Date de péremption: 12 months

Détail du antigène

Antigène: ICOSLG (Inducible T-Cell Co-Stimulator Ligand (ICOSLG))

Autre désignation: B7-H2

Sujet: B7-H2, also known as B7-related protein (B7RP1), ICOS Ligand, and CD275, is an approximately 60 kDa transmembrane glycoprotein in the B7 family of immune regulatory molecules. B7-H2 is a ligand for the T-cell-specific cell surface receptor ICOS. Acts as a costimulatory signal for T-cell proliferation and cytokine secretion, induces also B-cell proliferation and differentiation into plasma cells.

Poids moléculaire: 29.5 kDa. Due to glycosylation, the protein migrates to 50-70 kDa based on Bis-Tris PAGE result.

UniProt: O75144

Pathways: Production of Molecular Mediator of Immune Response, Activated T Cell Proliferation