GFRAL Protein (AA 19-351) (His-Avi Tag)

N° du produit ABIN7274737

Détail du produit

Antigène: GFRAL (GDNF Family Receptor alpha Like (GFRAL))

Type de proteíne: Recombinant

Attributs du protein: AA 19-351

Origine: Humain

Source: HEK-293 Cells

Purification/Conjugué: Cette GFRAL protéine est marqué à la His-Avi Tag.

Fonction: Human GFRAL/GFR alpha-like Protein

Séquence: Ser19-Glu351

Attributs du produit: Recombinant Human GFRAL/GFR alpha-like Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains Ser19-Glu351.

Pureté: > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC

Stérilité: 0.22 μm filtered

niveau d'endotoxine: Less than 1EU per μg by the LAL method.

Biological Activity Comment: Immobilized Human GFRAL, His Tag at 0.5μg/ml (100μl/Well). Dose response curve for Human GDF15, hFc Tag with the EC50 of 9.4ng/ml determined by ELISA. See testing image for detail.

Information d'application

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/mL is recommended. Dissolve the lyophilized protein in distilled water.

Buffer: Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4).Normally 8 % trehalose is added as protectant before lyophilization.

Stock: -20 °C,-80 °C

Stockage commentaire: -20 to -80°C for 12 months as supplied from date of receipt., -80°C for 3-6 months after reconstitution., 2-8°C for 2-7 days after reconstitution., Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Date de péremption: 12 months

Détail du antigène

Antigène: GFRAL (GDNF Family Receptor alpha Like (GFRAL))

Autre désignation: GFRAL (GFRAL Produits)

Synonymes: RGD1565220 Protein, C6orf144 Protein, GRAL Protein, UNQ9356 Protein, bA360D14.1 Protein, AY457637 Protein, Gral Protein, GDNF family receptor alpha like Protein, Gfral Protein, GFRAL Protein

Sujet: GFR alpha -like (GDNF receptor-alpha-like) is a distant member of the GDNFR family of proteins. Mature human GFR alpha-like is a 376 amino acid (aa) type I transmembrane protein. It contains a 333 aa extracellular domain, a 20 aa transmembrane domain and a 23 aa cytoplasmic domain.GFRAL is a brainstem-restricted receptor for GDF15 which regulates food intake, energy expenditure and body weight in response to metabolic and toxin-induced stresses.

Poids moléculaire: 40.7 kDa. Due to glycosylation, the protein migrates to 48-62 kDa based on Tris-Bis PAGE result.

UniProt: Q6UXV0