IgE Protein (AA 209-428) (His-Avi Tag)

N° du produit ABIN7274855

Détail du produit

Antigène: IgE

Type de proteíne: Recombinant

Attributs du protein: AA 209-428

Origine: Humain

Source: HEK-293 Cells

Purification/Conjugué: Cette IgE protéine est marqué à la His-Avi Tag.

Fonction: Human IgE Protein

Séquence: Cys209-Lys428

Attributs du produit: Recombinant Human IgE Protein is expressed from HEK293 with His and Avi tag at the C-Terminus.It contains Cys209-Lys428.

Pureté: > 95 % as determined by Tris-Bis PAGE

Stérilité: 0.22 μm filtered

niveau d'endotoxine: Less than 1EU per μg by the LAL method.

Biological Activity Comment: Immobilized Human IgE, His Tag at 0.5μg/ml (100μl/well) on the plate. Dose response curve for Anti-IgE Antibody, hFc Tag with the EC50 of 10.3ng/ml determined by ELISA. See testing image for detail.

Information d'application

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/mL is recommended. Dissolve the lyophilized protein in distilled water.

Buffer: Lyophilized from 0.22μm filtered solution in 20 mM PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.

Stock: -20 °C,-80 °C

Stockage commentaire: -20 to -80°C for 12 months as supplied from date of receipt.,-80°C for 3-6 months after reconstitution.,2-8°C for 2-7 days after reconstitution.,Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Date de péremption: 12 months

Détail du antigène

Antigène: IgE

Abstract: IgE Produits

Synonymes: IGH2 Protein, IgE Protein, Gm900 Protein, immunoglobulin heavy chain (epsilon polypeptide) Protein, immunoglobulin heavy constant epsilon Protein, Immunoglobulin heavy constant epsilon Protein, Ighe Protein, IGHE Protein

Sujet: Immunoglobulin E (IgE) is well known for its role in allergic disease, the manifestations of which are mediated through its two Fc receptors, FcεRI and CD23 (FcεRII). IgE and its interactions with these receptors are therefore potential targets for therapeutic intervention, and exciting progress has been made in this direction. Furthermore, recent structural studies of IgE-Fc, the two receptors, and of their complexes, have revealed a remarkable degree of plasticity at the IgE-CD23 interface and an even more remarkable degree of dynamic flexibility within the IgE molecule.

Poids moléculaire: 27.8 kDa. Due to glycosylation, the protein migrates to 30-35 kDa based on Tris-Bis PAGE result.

UniProt: P01854