IgG1 Fc (AA 104-330) Protéine

N° du produit ABIN7274867

Protéine Recombinant exprimée dans HEK-293 Cells.

Aperçu rapide pour IgG1 Fc (AA 104-330) Protéine (ABIN7274867)

Antigène: IgG1 Fc

Type de proteíne: Recombinant

Origine: Humain

Source: HEK-293 Cells

Pureté: > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC

Détail du produit

Attributs du protein: AA 104-330

Fonction: Human IgG1 Fc Protein

Séquence: Asp104-Lys330

Attributs du produit: Recombinant Human IgG1 Fc is expressed from HEK293 without tag.It contains Asp104-Lys330.

Stérilité: 0.22 μm filtered

niveau d'endotoxine: Less than 1EU per μg by the LAL method.

Biological Activity Comment: The affinity constant of 0.283 μM as determined in SPR assay (Biacore T200). See testing image for detail.

Information d'application

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/mL is recommended. Dissolve the lyophilized protein in distilled water.

Buffer: Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.

Stock: -20 °C,-80 °C

Stockage commentaire: -20 to -80°C for 12 months as supplied from date of receipt., -80°C for 3-6 months after reconstitution., 2-8°C for 2-7 days after reconstitution., Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Date de péremption: 12 months

Détail du antigène

Antigène: IgG1 Fc

Sujet: IgG1 is most abundant in serum among the four IgG subclasses (IgG1, 2, 3 and 4) and binds to Fc receptors (FcγR) on phagocytic cells with high affinity. Fc fragment is demonstrated to mediate phagocytosis, trigger inflammation, and target Ig to particular tissues.IgG1 Fc was reported has a novel role as a potential anti-inflammatory drug for treatment of human autoimmune diseases.

Poids moléculaire: 25.5 kDa. Due to glycosylation, the protein migrates to 31-33 kDa based on Tris-Bis PAGE result.

UniProt: P01857-1