IgG2a Fc (AA 98-330) (Active) Protéine

N° du produit ABIN7274869

Cette protéine Recombinant IgG2a Fc est exprimée dans HEK-293 Cells.

Aperçu rapide pour IgG2a Fc (AA 98-330) (Active) Protéine (ABIN7274869)

Antigène: IgG2a Fc

Type de proteíne: Recombinant

Activité biologique: Active

Origine: Souris

Source: HEK-293 Cells

Pureté: > 95 % as determined by Bis-Tris PAGE,> 95 % as determined by HPLC

Détail du produit

Attributs du protein: AA 98-330

Fonction: Mouse IgG2a Fc Protein

Séquence: Glu98-Lys330

Attributs du produit: Recombinant Mouse IgG2a Fc Protein is expressed from HEK293 without tag.It contains Glu98-Lys330.

Stérilité: 0.22 μm filtered

niveau d'endotoxine: Less than 1 EU per μg by the LAL method.

Biological Activity Comment: Mouse FcRn, His Tag captured on CM5 Chip via Anti-His Antibody can bind Mouse IgG2a Fc, No Tag with an affinity constant of 2.72 nM as determined in SPR assay (Biacore T200).,Mouse IgG2a Fc on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.,The purity of Mouse IgG2a Fc is greater than 95% as determined by SEC-HPLC.

Information d'application

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 μg or less, at 1 mg/mL for 500 μg or 1 mg lyophilized powder. Do not mix by vortex or vigorous shaking.

Buffer: Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.

Stock: -20 °C,-80 °C

Stockage commentaire: -20 to -80°C for 12 months as supplied from date of receipt.,-80°C for 3 months after reconstitution.,Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Date de péremption: 12 months

Détail du antigène

Antigène: IgG2a Fc

Sujet: Mouse IgG2a is one of the subtypes of murine immunoglobulin IgG which mediates immune responses to carbohydrate or polysaccharide antigens. The Fc region of IgG2a can bind to FcRn to prolong its half-life.

Poids moléculaire: 26.38 kDa. Due to glycosylation, the protein migrates to 33-38 kDa based on Bis-Tris PAGE result.

UniProt: P01863