IL-25 Protein (AA 33-177) (His tag)
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- Antigène Voir toutes IL-25 (IL25) Protéines
- IL-25 (IL25) (Interleukin 25 (IL25))
- Type de proteíne
- Recombinant
- Attributs du protein
- AA 33-177
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Origine
- Humain
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Source
- HEK-293 Cells
- Purification/Conjugué
- Cette IL-25 protéine est marqué à la His tag.
- Fonction
- Human IL-25/IL-17E Protein
- Séquence
- Tyr33-Gly177
- Attributs du produit
- Recombinant Human IL-25/IL-17E Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Tyr33-Gly177.
- Pureté
- > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
- Stérilité
- 0.22 μm filtered
- niveau d'endotoxine
- Less than 1EU per μg by the LAL method.
- Biological Activity Comment
- The affinity constant of 0.81nM as determined in SPR assay (Biacore T200). See testing image for detail.
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- Restrictions
- For Research Use only
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- Format
- Lyophilized
- Reconstitution
- Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/mL is recommended. Dissolve the lyophilized protein in distilled water.
- Buffer
- Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.
- Stock
- -20 °C,-80 °C
- Stockage commentaire
- -20 to -80°C for 12 months as supplied from date of receipt., -80°C for 3-6 months after reconstitution., 2-8°C for 2-7 days after reconstitution., Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
- Date de péremption
- 12 months
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- Antigène
- IL-25 (IL25) (Interleukin 25 (IL25))
- Autre désignation
- IL-25 (IL25 Produits)
- Synonymes
- IL17E Protein, IL-17e Protein, Il17e Protein, IL25 Protein, RGD1561632 Protein, interleukin 25 Protein, IL25 Protein, Il25 Protein
- Sujet
- IL-25, also known as IL-17E, is a member of the IL-17 cytokine family mostly produced by epithelial cells and innate immune cells. After binding to the IL-17RB/IL-17RA complex, IL-25 induces downstream signaling responses in epithelial cells and type 2 lymphocytes, which initiates, propagates, and sustains type 2 immunity. The function of IL-25 in allergic diseases such as asthma has been well established, and now also is extended to diseases such as inflammatory bowel disease and cancer.
- Poids moléculaire
- 17.6 kDa. Due to glycosylation, the protein migrates to 25-30 kDa based on Tris-Bis PAGE result.
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