IL-22 Protein (AA 34-179) (Biotin,His-Avi Tag)

N° du produit ABIN7274927

Protéine Recombinant IL-22 exprimée dans Escherichia coli (E. coli).

Aperçu rapide pour IL-22 Protein (AA 34-179) (Biotin,His-Avi Tag) (ABIN7274927)

Antigène: IL-22 (IL22) (Interleukin 22 (IL22))

Type de proteíne: Recombinant

Activité biologique: Active

Origine: Souris

Source: Escherichia coli (E. coli)

Pureté: > 95 % as determined by Bis-Tris PAGE,> 90 % as determined by HPLC

Détail du produit

Attributs du protein: AA 34-179

Purification/Conjugué: Cette IL-22 protéine est marqué à la Biotin,His-Avi Tag.

Fonction: Biotinylated Mouse IL-22 Protein

Séquence: Leu34-Val179

Attributs du produit: Recombinant Biotinylated Mouse IL-22 Protein is expressed from E.coli with His tag and Avi tag at the N-Terminus.It contains Leu34-Val179.

Stérilité: 0.22 μm filtered

niveau d'endotoxine: Less than 1 EU per μg by the LAL method.

Biological Activity Comment: Rat IL-22R alpha 1, His Tag immobilized on CM5 Chip can bind Biotinylated Mouse IL-22, His Tag with an affinity constant of 0.145 uM as determined in SPR assay (Biacore T200).,Immobilized Biotinylated Mouse IL-22, His Tag at 2ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Mouse IL-22R alpha 1, hFc Tag with the EC50 of 15.2ng/ml determined by ELISA (QC Test).,Biotinylated Mouse IL-22 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.,Loaded Mouse IL-22R alpha 1, hFc Tag on ProA-Biosensor can bind Biotinylated Mouse IL-22, His-Avi Tag with an affinity constant of 13.50 nM as determined in BLI assay (Gator® Prime).,The purity of Biotinylated Mouse IL-22 is greater than 90% as determined by SEC-HPLC.

Information d'application

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 μg or less, at 1 mg/mL for 500 μg or 1 mg lyophilized powder. Do not mix by vortex or vigorous shaking.

Buffer: Lyophilized from 0.22 μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.

Stock: -20 °C,-80 °C

Stockage commentaire: -20 to -80°C for 12 months as supplied from date of receipt.,-80°C for 3 months after reconstitution.,Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Date de péremption: 12 months

Détail du antigène

Antigène: IL-22 (IL22) (Interleukin 22 (IL22))

Autre désignation: IL-22

Sujet: The cytokine interleukin-22 (IL-22) is a critical regulator of epithelial homeostasis. It has been implicated in multiple aspects of epithelial barrier function, including regulation of epithelial cell growth and permeability, production of mucus and antimicrobial proteins (AMPs), and complement production.

Poids moléculaire: 19.7 kDa same as Bis-Tris PAGE result.

UniProt: Q9JJY9