IL-33 Protein (AA 112-270)

N° du produit ABIN7274939

Protéine Recombinant IL-33 exprimée dans Escherichia coli (E. coli).

Aperçu rapide pour IL-33 Protein (AA 112-270) (ABIN7274939)

Antigène: IL-33 (IL33) (Interleukin 33 (IL33))

Type de proteíne: Recombinant

Activité biologique: Active

Origine: Humain

Source: Escherichia coli (E. coli)

Pureté: > 95 % as determined by Bis-Tris PAGE,> 95 % as determined by HPLC

Détail du produit

Attributs du protein: AA 112-270

Fonction: Human IL-33 Protein

Séquence: Ser112-Thr270

Attributs du produit: Recombinant Human IL-33 Protein is expressed from E.coli without tag.It contains Ser112-Thr270.

Stérilité: 0.22 μm filtered

niveau d'endotoxine: Less than 1 EU per μg by the LAL method.

Biological Activity Comment: Immobilized Human IL-33, No Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Human IL-1RL1, His Tag with the EC50 of 7.4ng/ml determined by ELISA (QC Test).,Immobilized Human IL-33, No Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Anti-IL-33 Antibody, hFc Tag with the EC50 of 4.3ng/ml determined by ELISA.,Human IL-33 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.,The purity of Human IL-33 is greater than 95% as determined by SEC-HPLC.

Information d'application

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 μg or less, at 1 mg/mL for 500 μg or 1 mg lyophilized powder. Do not mix by vortex or vigorous shaking.

Buffer: Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.

Stock: -20 °C,-80 °C

Stockage commentaire: -20 to -80°C for 12 months as supplied from date of receipt.,-80°C for 3 months after reconstitution.,Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Date de péremption: 12 months

Détail du antigène

Antigène: IL-33 (IL33) (Interleukin 33 (IL33))

Autre désignation: IL-33

Sujet: Interleukin-33 (IL-33) is a cytokine belonging to the IL-1 family, playing a role in inflammatory, infectious and autoimmune diseases and expressed in the cellular nucleus in several tissues. High levels of IL-33 are expressed in epithelial barrier tissues and endothelial barriers. ST2 is a receptor for IL-33, expressed selectively on a subset of Th2 cells, mediating some of their functions.

Poids moléculaire: 18 kDa same as Bis-Tris PAGE result.

NCBI Accession: NP_254274

Pathways: Production of Molecular Mediator of Immune Response