NKG2A & CD94 (AA 100-233) protein (His-Avi Tag)

N° du produit ABIN7275328

Cette protéine Recombinant est exprimée dans HEK-293 Cells.

Aperçu rapide pour NKG2A & CD94 (AA 100-233) protein (His-Avi Tag) (ABIN7275328)

Antigène: NKG2A & CD94

Type de proteíne: Recombinant

Origine: Humain

Source: HEK-293 Cells

Pureté: > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC

Détail du produit

Attributs du protein: AA 100-233

Purification/Conjugué: His-Avi Tag

Fonction: Human NKG2A&CD94 Protein

Séquence: Arg100-Leu233(NKG2A) & Ser34-Ile179(CD94)

Attributs du produit: Recombinant Human NKG2A&CD94 Protein is expressed from HEK293 with His tag and Avi tag at the N-Terminus.It contains Arg100-Leu233(NKG2A)&Ser34-Ile179(CD94).

Stérilité: 0.22 μm filtered

niveau d'endotoxine: Less than 1EU per μg by the LAL method.

Biological Activity Comment: Immobilized Human NKG2A&CD94, His Tag at 0.5μg/ml (100μl/well) on the plate. Dose response curve for Anti-NKG2A Antibody, hFc Tag with the EC50 of 7.9ng/ml determined by ELISA. See testing image for detail.

Information d'application

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/mL is recommended. Dissolve the lyophilized protein in distilled water.

Buffer: Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.

Stock: -20 °C,-80 °C

Stockage commentaire: -20 to -80°C for 12 months as supplied from date of receipt., -80°C for 3-6 months after reconstitution., 2-8°C for 2-7 days after reconstitution., Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Date de péremption: 12 months

Détail du antigène

Antigène: NKG2A & CD94

Autre désignation: NKG2A&CD94

Sujet: The ligand-receptor assignment between HLA-G and NKG2A/CD94 is dependent of the amino acid composition in the HLA-G heavy chain. Understanding the biophysical basis of receptor-mediated events that lead to NK cell inhibition would help to remove non-tumor reactive cells and support personalized mild autologous NK cell therapies.

Poids moléculaire: 17.9 kDa (CD94)&18.3 kDa (NKG2A). Due to glycosylation, the protein migrates to 33-48 kDa based on Tris-Bis PAGE result.