PD-L1 Protein (AA 19-238) (His tag)

N° du produit ABIN7275429

Cette protéine Recombinant PD-L1 est produite dans HEK-293 Cells.

Aperçu rapide pour PD-L1 Protein (AA 19-238) (His tag) (ABIN7275429)

Antigène: PD-L1 (CD274 (PD-L1))

Type de proteíne: Recombinant

Activité biologique: Active

Origine: Humain

Source: HEK-293 Cells

Pureté: > 95 % as determined by Bis-Tris PAGE,> 95 % as determined by HPLC

Détail du produit

Attributs du protein: AA 19-238

Purification/Conjugué: Cette PD-L1 protéine est marqué à la His tag.

Fonction: Human PD-L1/B7-H1 Protein

Séquence: Phe19-Arg238

Attributs du produit: Recombinant Human PD-L1/B7-H1 Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Phe19-Arg238.

Stérilité: 0.22 μm filtered

niveau d'endotoxine: Less than 1 EU per μg by the LAL method.

Biological Activity Comment: Human PD-1, hFc Tag captured on CM5 Chip via Protein A can bind Human PD-L1, His Tag with an affinity constant of 0.25 μM as determined in SPR assay (Biacore T200).,Immobilized Human PD-L1, His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Anti-PD-L1 Antibody, hFc Tag with the EC50 of 0.92ng/ml determined by ELISA.,Human PD-L1 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.,Serial dilutions of Anti-PD-L1 Antibody were added into Human PD-L1, His Tag : Biotinylated Human PD-1, hFc Avi Tag binding reactioins. The half maximal inhibitiory concentration(IC50) is 11.7ng/ml.,Loaded Biotinylated Human PD-1, His Tag on Streptavidin-Biosensor can bind Human PD-L1, His Tag with an affinity constant of 0.20 μM as determined in BLI assay (Gator® Prime).,The purity of Human PD-L1 is greater than 95% as determined by SEC-HPLC.

Information d'application

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 μg or less, at 1 mg/mL for 500 μg or 1 mg lyophilized powder. Do not mix by vortex or vigorous shaking.

Buffer: Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.

Stock: -20 °C,-80 °C

Stockage commentaire: -20 to -80°C for 12 months as supplied from date of receipt.,-80°C for 3 months after reconstitution.,Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Date de péremption: 12 months

Détail du antigène

Antigène: PD-L1 (CD274 (PD-L1))

Autre désignation: PD-L1

Sujet: B7-H1, also known as PD-L1 and CD274, is an approximately 65 kDa transmembrane glycoprotein in the B7 family of immune regulatory molecules. PD-L1 has been identified as the ligand for the immunoinhibitory receptor programmed death-1(PD1/PDCD1) and has been demonstrated to play a role in the regulation of immune responses and peripheral tolerance.

Poids moléculaire: 26 kDa. Due to glycosylation, the protein migrates to 35-42 kDa based on Bis-Tris PAGE result.

UniProt: Q9NZQ7

Pathways: Cancer Immune Checkpoints