RSPO1 Protein (AA 21-265) (His tag)
Aperçu rapide pour RSPO1 Protein (AA 21-265) (His tag) (ABIN7275538)
Antigène
Voir toutes RSPO1 ProtéinesType de proteíne
Origine
Source
Pureté
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Attributs du protein
- AA 21-265
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Purification/Conjugué
- Cette RSPO1 protéine est marqué à la His tag.
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Fonction
- Mouse R spondin 1/RSPO1 Protein
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Séquence
- Ser21-Gln265
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Attributs du produit
- Recombinant Mouse R spondin 1/RSPO1 Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Ser21-Gln265.
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Stérilité
- 0.22 μm filtered
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niveau d'endotoxine
- Less than 1EU per μg by the LAL method.
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Biological Activity Comment
- Immobilized Mouse R spondin 1, His Tag at 2μg/ml (100μl/Well) on the plate. Dose response curve for Mouse RNF43, hFc Tag with the EC50 of 0.21μg/ml determined by ELISA. See testing image for detail.
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R-Spondin 1 (RSPO1) (AA 21-146) protein (His tag)
RSPO1 Origine: Humain Hôte: HEK-293 Cells Recombinant >95 % as determined by SDS-PAGE.
R-Spondin 1 (RSPO1) (Active) protein (His tag)RSPO1 Origine: Souris Hôte: CHO Cells Recombinant > 95 % as determined by SDS-PAGE Active
R-Spondin 1 (RSPO1) (AA 21-146) protein (Fc Tag)RSPO1 Origine: Humain Hôte: HEK-293 Cells Recombinant The purity of the protein is greater than 95 % as determined by SDS-PAGE and Coomassie blue staining.
R-Spondin 1 (RSPO1) (AA 21-263) protein (His tag)RSPO1 Origine: Humain Hôte: HEK-293 Cells Recombinant The purity of the protein is greater than 85 % as determined by SDS-PAGE and Coomassie blue staining.
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Restrictions
- For Research Use only
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Format
- Lyophilized
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Reconstitution
- Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/mL is recommended. Dissolve the lyophilized protein in 20 mM MES, 150 mM NaCl, 200 mM Arginine ( pH 5.5).
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Buffer
- Lyophilized from 0.22 μm filtered solution in 20 mM MES, 150 mM NaCl, 200 mM Arginine ( pH 5.5). Normally 8 % trehalose / 8 % mannitol is added as protectant before lyophilization.
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Stock
- -20 °C,-80 °C
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Stockage commentaire
- -20 to -80°C for 12 months as supplied from date of receipt.,-80°C for 3-6 months after reconstitution.,2-8°C for 2-7 days after reconstitution.,Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
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Date de péremption
- 12 months
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- RSPO1 (R-Spondin 1 (RSPO1))
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Autre désignation
- R spondin 1
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Sujet
- The R-spondins are members of a superfamily of thrombospondin type 1 repeat (TSR-1)-containing proteins. The prototype member (discovered in 1971) was isolated from platelets that had been stimulated with thrombin, and was therefore designated 'thrombin-sensitive protein.
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Poids moléculaire
- 28.3 kDa. Due to glycosylation, the protein migrates to 45-48 kDa based on Tris-Bis PAGE result.
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UniProt
- Q9Z132
Antigène
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