SARS-CoV-2 Spike S1 Protein (Fc-Avi Tag)
Aperçu rapide pour SARS-CoV-2 Spike S1 Protein (Fc-Avi Tag) (ABIN7275552)
Antigène
Voir toutes SARS-CoV-2 Spike S1 ProtéinesType de proteíne
Origine
Source
Pureté
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Purification/Conjugué
- Cette SARS-CoV-2 Spike S1 protéine est marqué à la Fc-Avi Tag.
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Fonction
- SARS Spike S1 Protein
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Séquence
- Ser14-Arg667
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Attributs du produit
- Recombinant SARS spike S1 protein is expressed from HEK293 with hFc tag and Avi tag at the C-Terminus.It contains Ser14-Arg667.
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Stérilité
- 0.22 μm filtered
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niveau d'endotoxine
- Less than 1EU per μg by the LAL method.
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Biological Activity Comment
- Immobilized Human ACE2 at 2μg/ml (100μl/Well).Dose response curve for SARS Spike S1, hFc Tag with the EC50 of 0.39μg/ml determined by ELISA. See testing image for detail.
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Restrictions
- For Research Use only
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Format
- Lyophilized
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Reconstitution
- Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/mL is recommended. Dissolve the lyophilized protein in distilled water.
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Buffer
- Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.
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Stock
- -20 °C,-80 °C
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Stockage commentaire
- -20 to -80°C for 12 months as supplied from date of receipt., -80°C for 3-6 months after reconstitution., 2-8°C for 2-7 days after reconstitution., Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
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Date de péremption
- 12 months
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- SARS-CoV-2 Spike S1
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Autre désignation
- SARS-CoV Spike S1
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Sujet
- The spike protein (S) of coronavirus (CoV) attaches the virus to its cellular receptor, angiotensin-converting enzyme 2 (ACE2). A defined receptor-binding domain (RBD) on S mediates this interaction.The S protein plays key parts in the induction of neutralizing-antibody and T-cell responses, as well as protective immunity.
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Poids moléculaire
- 100.6 kDa. Due to glycosylation, the protein migrates to 120-140 kDa based on Tris-Bis PAGE result.
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UniProt
- P59594
Antigène
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