Latent TGF-beta 1 (AA 30-390) protein (His tag)

N° du produit ABIN7275704

Protéine Recombinant exprimée dans HEK-293 Cells.

Aperçu rapide pour Latent TGF-beta 1 (AA 30-390) protein (His tag) (ABIN7275704)

Antigène: Latent TGF-beta 1

Type de proteíne: Recombinant

Origine: Macaque rhésus

Source: HEK-293 Cells

Pureté: > 95 % as determined by Tris-Bis PAGE

Détail du produit

Attributs du protein: AA 30-390

Purification/Conjugué: His tag

Fonction: Rhesus macaque Latent TGF beta 1/TGFB1 Protein

Séquence: Leu30-Ser390(C33S)

Attributs du produit: Recombinant Rhesus macaque Latent TGF beta 1/TGFB1 Protein is expressed from HEK293 with His tag at the N-Terminus.It contains Leu30-Ser390(C33S).

Stérilité: 0.22 μm filtered

niveau d'endotoxine: Less than 1EU per μg by the LAL method.

Biological Activity Comment: Immobilized Rhesus macaque Latent TGF beta 1, His Tag at 2μg/ml (100μl/Well) on the plate. Dose response curve for Biotinylated Human ITGAV&ITGB6, His Tag with the EC50 of 1.2μg/ml determined by ELISA. See testing image for detail.

Information d'application

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/mL is recommended. Dissolve the lyophilized protein in distilled water.

Buffer: Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.

Stock: -20 °C,-80 °C

Stockage commentaire: -20 to -80°C for 12 months as supplied from date of receipt., -80°C for 3-6 months after reconstitution., 2-8°C for 2-7 days after reconstitution., Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Date de péremption: 12 months

Détail du antigène

Antigène: Latent TGF-beta 1

Autre désignation: Latent TGF beta 1

Sujet: Latent TGF beta 1  cDNA encodes a 390 amino acid precursor that contains a 29 aa signal peptide and a 361 aa proprotein. A furin-like convertase processes the proprotein to generate an N-terminal 249 aa latency-associated peptide (LAP) and a C-terminal 112 aa mature TGF- beta 1. Disulfide-linked homodimers of LAP and TGF- beta 1 remain non-covalently associated after secretion, forming the small latent TGF- beta 1 complex.

Poids moléculaire: 31.4/12.8 kDa. Due to glycosylation, the protein migrates to 40-50/14-15 kDa based on Tris-Bis PAGE result.

UniProt: F7HCV5