SARS-CoV-2 Spike Protein (SARS-CoV-2 S) (AA 319-541, B.1.1.529 - Omicron, RBD) (Fc Tag)

N° du produit ABIN7490663

Cette protéine Recombinant SARS-CoV-2 Spike est exprimée dans HEK-293 Cells.

Aperçu rapide pour SARS-CoV-2 Spike Protein (SARS-CoV-2 S) (AA 319-541, B.1.1.529 - Omicron, RBD) (Fc Tag) (ABIN7490663)

Antigène: SARS-CoV-2 Spike (SARS-CoV-2 S)

Type de proteíne: Recombinant

Origine: SARS Coronavirus-2 (SARS-CoV-2)

Source: HEK-293 Cells

Application: Immunoassay (IA), Affinity Measurement (AM), Antibody Drug Assay (ADA), Screening Assay (ScA)

Pureté: The purity of the protein is greater than 95 % as determined by SDS-PAGE and Coomassie blue staining.

Détail du produit

Attributs du protein: AA 319-541, B.1.1.529 - Omicron, RBD

Purification/Conjugué: Cette SARS-CoV-2 Spike protéine est marqué à la Fc Tag.

Fonction: Recombinant SARS-CoV-2 RBD(G339D, S371L, S373P, S375F, K417N, N440K, G446S, S477N, T478K, E484A, Q493R, G496S, Q498R, N501Y, Y505H) protein with C-terminal human Fc tag

Specificité: S protein RBD(G339D, S371L, S373P, S375F, K417N, N440K, G446S, S477N, T478K, E484A, Q493R, G496S, Q498R, N501Y, Y505H)(Arg319-Phe541) hFc(Glu99-Ala330)

Attributs du produit: Extracellular Domain Protein

Purification: Purified from cell culture supernatant by affinity chromatography

Information d'application

Indications d'application: Extracellular Domain Proteins (ECD) can be used as:

• Immunogens for antibody drug development
• Reagents used for CAR-T positive cell monitoring
• Reagents for antibody screening and functional testing
• Reagents for antibody affinity measurement

Commentaires:

The protein was produced using HEK293 mammalian cell secretion expression system to ensure the close-to-native structures and post-translational modifications of the target protein.

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: Please see Certificate of Analysis for specific instructions of reconstitution.

Buffer: Lyophilized from sterile PBS, pH 7.4. Normally 5 % - 8 % trehalose is added as protectants before lyophilization.

Stock: -20 °C,-80 °C

Stockage commentaire: Store at -20°C to -80°C for 12 months in lyophilized form. After reconstitution, if not intended for use within a month, aliquot and store at -80°C (Avoid repeated freezing and thawing). Lyophilized proteins are shipped at ambient temperature.

Date de péremption: 12 months

Détail du antigène

Antigène: SARS-CoV-2 Spike (SARS-CoV-2 S)

Autre désignation: SARS-CoV-2 S protein

Classe de substances: Viral Protein

Sujet: SARS-CoV-2 B.1.1.529 (Omicron) Spike RBD Protein
SARS-CoV-2 (Severe Acute Respiratory Syndrome Coronavirus 2) also known as Covid19 (2019 Novel Coronavirus) is a virus that causes illnesses ranging from the common cold to severe diseases. The spike protein is a type I transmembrane protein containing two subunits, S1 and S2. S1 mainly contains a receptor binding domain (RBD), which accounts for recognizing the cell surface receptor, ACE2. S2 contains basic elements needed for the membrane fusion. Recent publications indicate that S1-RBD domain can induce virus neutralizing-antibody and T cell response.

Poids moléculaire: predicted molecular mass of 51.2 kDa after removal of the signal peptide.The apparent molecular mass of RBD(G339D, S371L, S373P, S375F, K417N, N440K, G446S, S477N, T478K, E484A, Q493R, G496S, Q498R, N501Y, Y505H)-hFc is 55-70 kDa due to glycosylation.

UniProt: P0DTC2