STEAP1 Protéine

N° du produit ABIN7491743

Protéine Synthetic Nanodisc STEAP1 exprimée dans HEK-293 Cells.

Aperçu rapide pour STEAP1 Protéine (ABIN7491743)

Antigène: STEAP1 (Six Transmembrane Epithelial Antigen of The Prostate 1 (STEAP1))

Type de proteíne: Synthetic Nanodisc

Origine: Humain

Source: HEK-293 Cells

Détail du produit

Fonction: Human STEAP1 full length protein-synthetic nanodisc

Attributs du produit: Unlike other membrane scaffold protein (MSP) Nanodisc on the market, our synthetic Nanodisc can be prepared directly from the cells. The polymers used during this process have a dual function. It dissolves the cell membranes, like the detergent, and uses cellular phospholipids to form Nanodisc around the membrane proteins. The target protein embedded Nanodiscs can then be purified.

Information d'application

Commentaires:

Advantages of Synthetic Nanodiscs:

• Highly purified membrane proteins
• High solubility in aqueous solutions
• High stability
• Proteins are in a native membrane environment and remain biologically active
• No detergent and can be used for cell-based assays
• No MSP backbone proteins

Limitations of Synthetic Nanodiscs:

• Intolerant to acids and high concentrations of divalent metal ions

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Buffer: Lyophilized from nanodisc solubilization buffer (20 mM Tris-HCl, 150 mM NaCl, pH 8.0). Normally 5 % - 8 % trehalose is added as protectants before lyophilization.

Stock: -20 °C,-80 °C

Stockage commentaire: Store at -20°C to -80°C for 12 months in lyophilized form. After reconstitution, if not intended for use within a month, aliquot and store at -80°C (Avoid repeated freezing and thawing). Lyophilized proteins are shipped at ambient temperature.

Date de péremption: 12 months

Détail du antigène

Antigène: STEAP1 (Six Transmembrane Epithelial Antigen of The Prostate 1 (STEAP1))

Autre désignation: STEAP1

Sujet: STEAP1 is a cell-surface biomolecule composed by sixtransmembrane domains connected by intra- and extracellular loops. It is commonly found overexpressed in several types of cancers, namely in PCa, and is preferentially located at the tight or gap junctions. However, in nontumoural tissues and vital organs, STEAP1 protein presents low or absent expression, unveiling considerable specificity for cancer environment. Taking into account STEAP1 predicted transmembrane topology and cellular localization, it has been hypothesized that STEAP1 may play an important role as a transporter protein and can be involved in intercellular communication.

Poids moléculaire: The human full length STEAP1 protein has a MW of 39.9 kDa

UniProt: Q9UHE8

Pathways: Transition Metal Ion Homeostasis