G Protein-Coupled Receptor 133 Protein (GPR133)

N° du produit ABIN7491529

Cette protéine Synthetic Nanodisc G Protein-Coupled Receptor 133 est exprimée dans HEK-293 Cells.

Aperçu rapide pour G Protein-Coupled Receptor 133 Protein (GPR133) (ABIN7491529)

Antigène: G Protein-Coupled Receptor 133 (GPR133)

Type de proteíne: Synthetic Nanodisc

Origine: Humain

Source: HEK-293 Cells

Détail du produit

Fonction: Human ADGRD1 full length protein-synthetic nanodisc

Attributs du produit: Unlike other membrane scaffold protein (MSP) Nanodisc on the market, our synthetic Nanodisc can be prepared directly from the cells. The polymers used during this process have a dual function. It dissolves the cell membranes, like the detergent, and uses cellular phospholipids to form Nanodisc around the membrane proteins. The target protein embedded Nanodiscs can then be purified.

Information d'application

Commentaires:

Advantages of Synthetic Nanodiscs:

• Highly purified membrane proteins
• High solubility in aqueous solutions
• High stability
• Proteins are in a native membrane environment and remain biologically active
• No detergent and can be used for cell-based assays
• No MSP backbone proteins

Limitations of Synthetic Nanodiscs:

• Intolerant to acids and high concentrations of divalent metal ions

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Buffer: Lyophilized from nanodisc solubilization buffer (20 mM Tris-HCl, 150 mM NaCl, pH 8.0). Normally 5 % - 8 % trehalose is added as protectants before lyophilization.

Stock: -20 °C,-80 °C

Stockage commentaire: Store at -20°C to -80°C for 12 months in lyophilized form. After reconstitution, if not intended for use within a month, aliquot and store at -80°C (Avoid repeated freezing and thawing). Lyophilized proteins are shipped at ambient temperature.

Date de péremption: 12 months

Détail du antigène

Antigène: G Protein-Coupled Receptor 133 (GPR133)

Autre désignation: ADGRD1

Sujet: The adhesion G-protein-coupled receptors (GPCRs), including GPR133, are membrane-bound proteins with long N termini containing multiple domains. GPCRs, or GPRs, contain 7 transmembrane domains and transduce extracellular signals through heterotrimeric G proteins.

Poids moléculaire: The human full length ADGRD1 protein has a MW of 96.5 kDa

UniProt: Q6QNK2