MHC, Class I Protein (AA 24-297, Monomer) (His-Avi Tag,Ovalbumin)

N° du produit ABIN7504348

Cette protéine Recombinant MHC, Class I est exprimée dans HEK-293 Cells.

Aperçu rapide pour MHC, Class I Protein (AA 24-297, Monomer) (His-Avi Tag,Ovalbumin) (ABIN7504348)

Antigène: MHC, Class I

Type de proteíne: Recombinant

Origine: Souris

Source: HEK-293 Cells

Pureté: > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC

Détail du produit

Attributs du protein: AA 24-297, Monomer

Purification/Conjugué: Cette MHC, Class I protéine est marqué à la His-Avi Tag,Ovalbumin.

Fonction: Mouse H-2K(b)&B2M&OVA (SIINFEKL) Monomer Protein

Séquence: His24-Pro297(H-2K(b)), Ile21-Met119(B2M) and SIINFEKL peptide

Specificité: Uni-Prot: P01901 (H-2K (B)), P01887 (B2M), SIINFEKL

Attributs du produit: Recombinant Mouse H-2K(b)&B2M&OVA (SIINFEKL) Monomer Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains His24-Pro297 (H-2K(b)), Ile21-Met119(B2M) and SIINFEKL peptide.

Stérilité: 0.22 μm filtered

niveau d'endotoxine: Less than 1EU per μg by the LAL method.

Information d'application

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/mL is recommended. Dissolve the lyophilized protein in distilled water.

Buffer: Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.

Stock: -20 °C,-80 °C

Stockage commentaire: -20 to -80°C for 12 months as supplied from date of receipt.,-80°C for 3-6 months after reconstitution.,2-8°C for 2-7 days after reconstitution.,Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Date de péremption: 12 months

Détail du antigène

Antigène: MHC, Class I

Autre désignation: H-2K

Sujet: Ovalbumin (OVA) has been historically a popular source of such antigens, since OVA can induce both humoral and cellular immune responses based on well-characterised peptide epitopes. The OVA257-264 octapeptide was one of the frst OVA epitopes to be characterised, it has an amino acid sequence SIINFEKL, which is recognised by cytotoxic T lymphocytes. SIINFEKL forms fbrillar assemblies similar to other peptide hydrogels. Te immunoactive properties of this peptide can therefore be related to its self-assembling nature.

Poids moléculaire: 50.20 kDa. Due to glycosylation, the protein migrates to 52-70 kDa based on Tris-Bis PAGE result.

UniProt: P01901