HLA-A*11:01 (AA 25-305), (Monomer) protein (His-Avi Tag,no Peptide)
Aperçu rapide pour HLA-A*11:01 (AA 25-305), (Monomer) protein (His-Avi Tag,no Peptide) (ABIN7504407)
Antigène
Type de proteíne
Origine
Source
Pureté
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Attributs du protein
- AA 25-305, Monomer
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Purification/Conjugué
- His-Avi Tag,no Peptide
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Fonction
- Human No Peptide HLA-A*11:01&B2M Monomer Protein
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Séquence
- Gly25-Thr305(HLA-A*11:01) & Ile21-Met119(B2M)
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Specificité
- Uni-Prot: AAV53343.1 (HLA-A*11:01), P61769 (B2M)
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Attributs du produit
- Recombinant Human No Peptide HLA-A*11:01&B2M Monomer Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains Gly25-Thr305(HLA-A*11:01) and Ile21-Met119(B2M).
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Stérilité
- 0.22 μm filtered
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niveau d'endotoxine
- Less than 1EU per μg by the LAL method.
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Indications d'application
- Can be used for Assess MHC/TCR Binding Affinity, generate custom MHC peptide monomer or tetramer, High Throughput Peptide Screening
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Commentaires
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The HLA-A*11:01&B2M Monomer is absent from peptide, namely peptide-receptive MHC. It can be loaded with antigenic peptides matching HLA-A*11:01. The MHC molecules comprising human HLA alleles and B2M, which can be readily tetramerized and loaded with peptides of choice in a high-throughput manner.
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Restrictions
- For Research Use only
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Format
- Lyophilized
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Reconstitution
- Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/mL is recommended. Dissolve the lyophilized protein in distilled water.
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Buffer
- Lyophilized from 0.22 μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.
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Stock
- -20 °C,-80 °C
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Stockage commentaire
- -20 to -80°C for 12 months as supplied from date of receipt., -80°C for 3-6 months after reconstitution., 2-8°C for 2-7 days after reconstitution., Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
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Date de péremption
- 12 months
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- HLA-A*11:01
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Sujet
- No Peptide HLA-A*11:01&B2M Monomer is absent from peptide, namely peptide-receptive MHC. It can be loaded with antigenic peptides matching HLA-A*11:01. No Peptide MHC molecules comprising human HLA alleles and B2M, which can be readily tetramerized and loaded with peptides of choice in a high-throughput manner.
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Poids moléculaire
- 48.50 kDa. Due to glycosylation, the protein migrates to 50-60 kDa based on Tris-Bis PAGE result.
Antigène
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