HLA-E*01:03 (AA 25-305), (Monomer) protein (Biotin,His-Avi Tag,no Peptide)

N° du produit ABIN7504416

Cette protéine Recombinant est produite dans HEK-293 Cells.

Aperçu rapide pour HLA-E*01:03 (AA 25-305), (Monomer) protein (Biotin,His-Avi Tag,no Peptide) (ABIN7504416)

Antigène: HLA-E*01:03

Type de proteíne: Recombinant

Origine: Humain

Source: HEK-293 Cells

Pureté: > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC

Détail du produit

Attributs du protein: AA 25-305, Monomer

Purification/Conjugué: Biotin,His-Avi Tag,no Peptide

Fonction: Biotinylated Human No Peptide HLA-E*01:03&B2M Monomer Protein

Séquence: Gly25-Ile305(HLA-E*01:03) & Ile21-Met119(B2M)

Specificité: Uni-Prot: P13747 (HLA-E*01:03), P61769 (B2M)

Attributs du produit: Recombinant Biotinylated Human No Peptide HLA-E*01:03&B2M Monomer Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains Gly25-Ile305(HLA-E*01:03) and Ile21-Met119(B2M).

Stérilité: 0.22 μm filtered

niveau d'endotoxine: Less than 1EU per μg by the LAL method.

Information d'application

Indications d'application: Can be used for Assess MHC/TCR Binding Affinity, generate custom MHC peptide monomer or tetramer, High Throughput Peptide Screening

Commentaires:

The HLA-E*01:03&B2M Monomer is absent from peptide, namely peptide-receptive MHC. It can be loaded with antigenic peptides matching HLA-E*01:03. The MHC molecules comprising human HLA alleles and B2M, which can be readily tetramerized and loaded with peptides of choice in a high-throughput manner.

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/mL is recommended. Dissolve the lyophilized protein in distilled water.

Buffer: Lyophilized from 0.22 μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.

Stock: -20 °C,-80 °C

Stockage commentaire: -20 to -80°C for 12 months as supplied from date of receipt., -80°C for 3-6 months after reconstitution., 2-8°C for 2-7 days after reconstitution., Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Date de péremption: 12 months

Détail du antigène

Antigène: HLA-E*01:03

Sujet: No Peptide HLA-E*01:03&B2M Monomer is absent from peptide, namely peptide-receptive MHC. It can be loaded with antigenic peptides matching HLA-E*01:03. No Peptide MHC molecules comprising human HLA alleles and B2M, which can be readily tetramerized and loaded with peptides of choice in a high-throughput manner.

Poids moléculaire: 48.30 kDa. Due to glycosylation, the protein migrates to 50-60 kDa based on Tris-Bis PAGE result.

UniProt: P13747