PD-L1 Protein (His tag)

N° du produit ABIN7763807

Cette protéine Recombinant PD-L1 est exprimée dans HEK-293 Cells.

Aperçu rapide pour PD-L1 Protein (His tag) (ABIN7763807)

Antigène: PD-L1 (CD274 (PD-L1))

Type de proteíne: Recombinant

Activité biologique: Active

Origine: Souris

Source: HEK-293 Cells

Pureté: > 95 % by SDS-PAGE.

Détail du produit

Purification/Conjugué: Cette PD-L1 protéine est marqué à la His tag.

Fonction: Active Recombinant Mouse B7-H1/PD-L1/CD274 Protein

Séquence: MRIFAGIIFT ACCHLLRAFT ITAPKDLYVV EYGSNVTMEC RFPVERELDL LALVVYWEKE DEQVIQFVAG EEDLKPQHSN FRGRASLPKD QLLKGNAALQ ITDVKLQDAG VYCCIISYGG ADYKRITLKV NAPYRKINQR ISVDPATSEH ELICQAEGYP EAEVIWTNSD HQPVSGKRSV TTSRTEGMLL NVTSSLRVNA TANDVFYCTF WRSQPGQNHT AELIIPELPA THPPQNRT

Specificité: Met1-Thr238

Stérilité: 0.22 μm filtered

niveau d'endotoxine: < 0.1 EU/μg of the protein by LAL method.

Biological Activity Comment: Measured by its binding ability in a functional ELISA. Immobilized Recombinant Mouse PD-L1 at 10 μg/mL (100 μL/well) can bind Recombinant Mouse PD-1 with a linear range of 1.5-5 μg/mL.

Information d'application

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: Centrifuge the vial before opening. Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. Avoid votex or vigorously pipetting the protein. For long term storage, it is recommended to add a carrier protein or stablizer (e.g. 0.1 % BSA, 5 % HSA, 10 % FBS or 5 % Trehalose), and aliquot the reconstituted protein solution to minimize free-thaw cycles.

Buffer: Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4.

Stock: -20 °C,-80 °C

Stockage commentaire: Store the lyophilized protein at -20°C to -80 °C for long term.
After reconstitution, the protein solution is stable at -20 °C for 3 months, at 2-8 °C for up to 1 week.

Détail du antigène

Antigène: PD-L1 (CD274 (PD-L1))

Autre désignation: B7-H1/PD-L1/CD274

Sujet:

Description: Programmed death-1 ligand-1 (PD-L1, CD274, B7-H1) has been identified as the ligand for the immunoinhibitory receptor programmed death-1(PD1/PDCD1). PD-L1/B7-H1 is expressed by hematopoietic and non-hematopoietic cells, such as T cells and B cells and various types of tumor cells. PD-L1/B7-H1 is a member of the growing B7 family of immune molecules that has immunoglobulin V-like and C-like domains. Interaction of this ligand with its receptor inhibits T-cell activation and cytokine production. During infection or inflammation of normal tissue, this interaction is important for preventing autoimmunity by maintaining homeostasis of the immune response. In tumor microenvironments, this interaction provides an immune escape for tumor cells through cytotoxic T-cell inactivation. Expression of this gene in tumor cells is considered to be prognostic in many types of human malignancies, including colon cancer and renal cell carcinoma.

Name: PD-, B7h1, Pdl1, Pdcd1l, Pdcd1l1, Pdcd1lg1, A530045L16Rik,CD274

ID gène: 60533

UniProt: Q9EP73

Pathways: Cancer Immune Checkpoints