Nuclease Protéine

N° du produit ABIN7520371

Cette protéine Recombinant Nuclease est produite dans Yeast (Pichia pastoris).

Aperçu rapide pour Nuclease Protéine (ABIN7520371)

Antigène: Nuclease

Type de proteíne: Recombinant

Activité biologique: Active

Origine: Serratia marcescens

Source: Yeast (Pichia pastoris)

Pureté: > 95 % by SDS-PAGE.

Détail du produit

Fonction: Active Recombinant Serratia marcescens Nuclease

Séquence: DTLESIDNCA VGCPTGGSSN VSIVRHAYTL NNNSTTKFAN WVAYHITKDT PASGKTRNWK TDPALNPADT LAPADYTGAN AALKVDRGHQ APLASLAGVS DWESLNYLSN ITPQKSDLNQ GAWARLEDQE RKLIDRADIS SVYTVTGPLY ERDMGKLPGT QKAHTIPSAY WKVIFINNSP AVNHYAAFLF DQNTPKGADF CQFRVTVDEI EKRTGLIIWA GLPDDVQASL KSKPGVLPEL MGCKN

Specificité: Asp22-Asn266

niveau d'endotoxine: <0.1EU/μg

Biological Activity Comment: 1.One unit (U) is defined as the amount of enzyme required to change the absorption value of A260 by 1.0 (equivalent to complete digestion of 37 μg of salmon essence DNA into oligonucleotides) in 30 min at 37°C, pH 8.0 reaction conditions. The specific activity of Serratia marcescens nuclease is > 1000 U/μL.|2.Recombinant Serratia marcescens Nuclease (0.1U) can effectively degrade 5 μg plasmid DNA at 37°C for 30 min. The reaction buffer is: 10 mM MgCl2, 0.1 mg/mL BSA, 50 mM Tris-HCl, pH8.5.

Information d'application

Restrictions: For Research Use only

Stockage

Format: Liquid

Buffer: Solution in 50 % glycerol containing 20 mM Tris HCl, pH 8.0, 2 mM MgCl₂, and 20 mM NaCl.

Stock: -20 °C

Stockage commentaire: This product is stable at -15°C ~ -25°C for up to 2 year from the date of receipt.|For optimal storage, aliquot into smaller quantities after centrifugation and store at recommended temperature.

Détail du antigène

Antigène: Nuclease

Sujet:

Description: Catalyzes the hydrolysis of both DNA and RNA, double- or single-stranded, at the 3'position of the phosphodiester bond to produce 5'-phosphorylated mono-, di-, tri- and tetranucleotides. DNA is a slightly better substrate than RNA.

Name: Endonuclease, Nuclease, nucA, nuc

ID gène: 66715254

UniProt: P13717