GPR109B Protéine

N° du produit ABIN7538325

Cette protéine Synthetic Nanodisc GPR109B est exprimée dans Mammalian Cells.

Aperçu rapide pour GPR109B Protéine (ABIN7538325)

Antigène: GPR109B (HCAR3) (Hydroxycarboxylic Acid Receptor 3 (HCAR3))

Type de proteíne: Synthetic Nanodisc

Origine: Humain

Source: Mammalian Cells

Détail du produit

Fonction: Human HCAR3 full length protein-synthetic nanodisc

Attributs du produit: Unlike other membrane scaffold protein (MSP) Nanodisc on the market, our synthetic Nanodisc can be prepared directly from the cells. The polymers used during this process have a dual function. It dissolves the cell membranes, like the detergent, and uses cellular phospholipids to form Nanodisc around the membrane proteins. The target protein embedded Nanodiscs can then be purified.

Information d'application

Commentaires:

Advantages of Synthetic Nanodiscs:

• Highly purified membrane proteins
• High solubility in aqueous solutions
• High stability
• Proteins are in a native membrane environment and remain biologically active
• No detergent and can be used for cell-based assays
• No MSP backbone proteins

Limitations of Synthetic Nanodiscs:

• Intolerant to acids and high concentrations of divalent metal ions

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Buffer: Lyophilized from nanodisc solubilization buffer (20 mM Tris-HCl, 150 mM NaCl, pH 8.0). Normally 5 % - 8 % trehalose is added as protectants before lyophilization.

Stock: -20 °C,-80 °C

Stockage commentaire: Store at -20°C to -80°C for 12 months in lyophilized form. After reconstitution, if not intended for use within a month, aliquot and store at -80°C (Avoid repeated freezing and thawing). Lyophilized proteins are shipped at ambient temperature.

Date de péremption: 12 months

Détail du antigène

Antigène: GPR109B (HCAR3) (Hydroxycarboxylic Acid Receptor 3 (HCAR3))

Autre désignation: HCAR3

Sujet: Receptor for 3-OH-octanoid acid mediates a negative feedback regulation of adipocyte lipolysis to counteract prolipolytic influences under conditions of physiological or pathological increases in beta-oxidation rates. Acts as a low affinity receptor for nicotinic acid. This pharmacological effect requires nicotinic acid doses that are much higher than those provided by a normal diet.[UniProtKB/Swiss-Prot Function]

Poids moléculaire: The human full length HCAR3 protein has a MW of 44.5kDa

UniProt: P49019