S1PR1 Protéine

N° du produit ABIN7538533

Protéine Synthetic Nanodisc S1PR1 exprimée dans Mammalian Cells.

Aperçu rapide pour S1PR1 Protéine (ABIN7538533)

Antigène: S1PR1 (Sphingosine-1-Phosphate Receptor 1 (S1PR1))

Type de proteíne: Synthetic Nanodisc

Origine: Humain

Source: Mammalian Cells

Détail du produit

Fonction: Human S1PR1 full length protein-synthetic nanodisc

Attributs du produit: Unlike other membrane scaffold protein (MSP) Nanodisc on the market, our synthetic Nanodisc can be prepared directly from the cells. The polymers used during this process have a dual function. It dissolves the cell membranes, like the detergent, and uses cellular phospholipids to form Nanodisc around the membrane proteins. The target protein embedded Nanodiscs can then be purified.

Information d'application

Commentaires:

Advantages of Synthetic Nanodiscs:

• Highly purified membrane proteins
• High solubility in aqueous solutions
• High stability
• Proteins are in a native membrane environment and remain biologically active
• No detergent and can be used for cell-based assays
• No MSP backbone proteins

Limitations of Synthetic Nanodiscs:

• Intolerant to acids and high concentrations of divalent metal ions

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Buffer: Lyophilized from nanodisc solubilization buffer (20 mM Tris-HCl, 150 mM NaCl, pH 8.0). Normally 5 % - 8 % trehalose is added as protectants before lyophilization.

Stock: -20 °C,-80 °C

Stockage commentaire: Store at -20°C to -80°C for 12 months in lyophilized form. After reconstitution, if not intended for use within a month, aliquot and store at -80°C (Avoid repeated freezing and thawing). Lyophilized proteins are shipped at ambient temperature.

Date de péremption: 12 months

Détail du antigène

Antigène: S1PR1 (Sphingosine-1-Phosphate Receptor 1 (S1PR1))

Autre désignation: S1PR1

Sujet: The protein encoded by this gene is structurally similar to G protein-coupled receptors and is highly expressed in endothelial cells. It binds the ligand sphingosine-1-phosphate with high affinity and high specificity, and suggested to be involved in the processes that regulate the differentiation of endothelial cells. Activation of this receptor induces cell-cell adhesion. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Mar 2016]

Poids moléculaire: The human full length S1PR1 protein has a MW of 42.8kDa

UniProt: P21453

Pathways: Signaling Events mediated by VEGFR1 and VEGFR2