Polystreptavidin Protéine

N° du produit ABIN7567110

Cette protéine Native Polystreptavidin est exprimée dans Streptomyces avidinii.

Aperçu rapide pour Polystreptavidin Protéine (ABIN7567110)

Antigène: Polystreptavidin (Poly Streptavidin)

Type de proteíne: Native

Origine: Streptomyces avidinii

Source: Streptomyces avidinii

Application: Functional Studies (Func), Lateral Flow (LF)

Détail du produit

Fonction: Polymerized Streptavidin

Attributs du produit: Polystreptavidin is a chemically modified, polymerized form of Streptavidin distinguished by its exceptionally high biotin-binding capacity. When applied as a coating on solid surfaces, Polystreptavidin provides a versatile platform for immobilizing and detecting a wide range of biotinylated molecules—including proteins, peptides, PCR fragments, haptens, and more.

These coatings combine outstanding binding efficiency with excellent chemical and thermal stability, along with an extended shelf life. Polystreptavidin is ideal for use on various substrates such as membranes, beads, biochips, and plastic materials, offering a robust and reliable solution for diverse analytical and diagnostic applications.

Purification: Dialysis

Information d'application

Indications d'application: Lateral Flow Assay (LFA): 2 mg/mL in PBS

Commentaires:

Binding affinity: Ka = 1015 M-1

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 5 mg/mL

Buffer: 5 mM PBS, 0.09% Sodium azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Cloudy suspension - mix well before use! Dilute to 2 mg/mL in PBS + 1% Sucrose and stripe at 1 μL/cm. Cure striped membrane in a sealed container or pouch with desiccant at 45°C for 24 hours.

Stock: 4 °C

Stockage commentaire: 2 to 8 °C. Do not freeze!

Date de péremption: 6 months

Détail du antigène

Antigène: Polystreptavidin (Poly Streptavidin)

Autre désignation: PolyStreptavidin

Sujet: Streptavidin is a tetrameric protein, composed of four subunits of approximately 13 kDa each, that exhibits an exceptionally strong affinity for the small molecule biotin. This interaction ranks among the most robust non-covalent bonds in biology and remains stable under extreme conditions such as high temperatures, varying pH levels, and exposure to solvents or other chemicals. Biotin can effectively outcompete most other ligands for streptavidin binding and can be easily linked to a variety of biomolecules, making this system highly versatile and widely used—especially in immunoassay applications.

A single streptavidin molecule is capable of binding up to four biotin or biotinylated proteins. However, the use of Polystreptavidin allows for the attachment of significantly more biotin molecules per complex. This enhanced binding capacity can lead to lower background noise, improved signal strength and specificity, and reduced reagent costs during the development and optimization of immunoassays.