alpha-Dendrotoxin Protéine

N° du produit ABIN7821944

Protéine Native alpha-Dendrotoxin exprimée dans Snake (Serpentes).

Aperçu rapide pour alpha-Dendrotoxin Protéine (ABIN7821944)

Antigène: alpha-Dendrotoxin

Type de proteíne: Native

Origine: Dendroaspis angusticeps

Source: Snake (Serpentes)

Application: Blocking Peptide (BP)

Pureté: ≥98 % (HPLC)

Détail du produit

Fonction: A Blocker of KV1.1, KV1.2, KV1.6 and ASIC Channels

Séquence: QPRRKLCILH RNPGRCYDKI PAFYYNQKKK QCERFDWSGC GGNSNRFKTI EECRRTCIG-OH

Specificité: Toxin target: KV1.1, KV1.2, KV1.6, ASIC channels,
Disulfide bonds between: Cys7-Cys57, Cys16-Cys40 and Cys32-Cys53 Gln1 - Pyrrolidone carboxylic acid

Attributs du produit: A Blocker of KV1.1, KV1.2, KV1.6 and ASIC Channels

Information d'application

Indications d'application: Antigen Preadsorption Control: 10 - 500 nM

Commentaires:

Electrophysiology

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: Centrifuge the vial (10,000 x g for 5 minutes) before adding solvent to spin down all the powder to the bottom of the vial. The lyophilized product may be difficult to visualize. Add solvent directly to the centrifuged vial. Gently tap, tilt, and roll the vial to aid dissolution. Avoid vigorous vortexing, light vortexing for up to 3 seconds is acceptable if needed. The product is soluble in pure water at high micromolar concentrations (100 μM - 1 mM). For long-term storage in solution, we recommend preparing a stock solution by dissolving the product in double-distilled water (ddH2O) at a concentration between 100-1000x of the final working concentration. Divide the stock solution into small aliquots and store at -20 °C. Before use, thaw the relevant vial(s) and dilute to the desired working concentration in your working buffer. Centrifuge all product preparations before use. It is recommended to prepare fresh solutions in working buffers just before use. Avoid multiple freeze-thaw cycles to maintain biological activity.

Buffer: Lyophilized from double distilled water (ddH2O). May contain TFA as a residual counter ion.

Stock: 4 °C,-20 °C

Stockage commentaire: The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods (up to 6 months), small aliquots should be stored at -20°C. We do not recommend storing the product in working solutions for longer than a few days. Avoid multiple freeze-thaw cycles.,The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods (up to 6 months), small aliquots should be stored at -20°C. We do not recommend storing the product in working solutions for longer than a few days. Avoid multiple freeze-thaw cycles.

Détail du antigène

Antigène: alpha-Dendrotoxin

Sujet: Venom basic protease inhibitor 1 homolog, Toxin C13S2C3, α-DTX,α-Dendrotoxin is isolated from Dendroaspis angusticeps snake venom by modification of the procedures of Harvey1 and Benishin2 and purified to homogeneity. α-Dendrotoxin blocks KV1.1 and KV1.2 channels (IC50= 0.4 to 4 and 1.1 to 12 nM in oocytes respectively, with higher values for mammalian cells).4 In addition, the toxin was shown to block KV1.6 (IC50= 9-25 nM).4α-Dendrotoxin was recently found to block ASIC currents in rat dorsal root ganglia with IC50 in the nM range5.

Poids moléculaire: 7047 Da

UniProt: P00980