NGF 2.5S (Active) protein (Atto 647N)

N° du produit ABIN7582347

Cette protéine Native NGF 2.5S est exprimée dans Souris.

Aperçu rapide pour NGF 2.5S (Active) protein (Atto 647N) (ABIN7582347)

Antigène: NGF 2.5S

Type de proteíne: Native

Activité biologique: Active

Origine: Souris

Source: Souris

Application: Cell Culture (CC), Cytotoxicity Test (CyTox), Fluorescence Microscopy (FM), Immunofluorescence (IF), Live Cell Imaging (LCI), Binding Studies (Bind), Flow Cytometry (FACS)

Détail du produit

Purification/Conjugué: Atto 647N

Fonction: mouse NGF 2.5S-ATTO Fluor-647N

Specificité: Toxin target: p75 neurotrophin receptor (p75NTR), TrkA receptors

Attributs du produit: ATTO 647N (Ex/Em: 646/664 nm). The extent of labeling is one molecule of dye per one mouse NGF 2.5S molecule, linked via primary amines.

Biological Activity Comment: The biological effects of NGF are mediated by two receptors: TrkA, which is specific for NGF, and p75NTR, which binds all the neurotrophins.

Information d'application

Indications d'application: Antigen Preadsorption Control: 4-100 nM

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: Centrifuge the vial at 10,000 x g for 5 minutes before opening to ensure all material is collected at the bottom. The lyophilized product may be difficult to visualize. Add solvent directly to the centrifuged vial and dissolve by gently tapping, tilting, or rolling the vial. Avoid vigorous vortexing, a brief, light vortex of 1-2 seconds is acceptable if necessary. Note: The product is typically supplied in a 0.5 mL conical vial. The product is readily soluble in high-quality pure water at micromolar to millimolar concentrations (typically 5 μM to 1 mM). For long-term storage in solution, we recommend preparing a stock solution by dissolving the product in double-distilled water (ddH2O) at a concentration between 100-1000x the final working concentration. Divide the stock solution into small, single-use aliquots and store at -20 °C. Before use, thaw the relevant vial(s) and dilute to the desired working concentration in your working buffer. Centrifuge all product preparations before use. It is recommended to prepare fresh working solutions just before use. Avoid multiple freeze-thaw cycles and exposure to light to maintain optimal biological activity.

Buffer: Lyophilized from double distilled water (ddH2O). May contain residual trifluoroacetate (TFA).

Conseil sur la manipulation: Centrifuge the vial at 10,000 x g for 5 minutes before opening to ensure all material is collected at the bottom,Avoid vigorous vortexing. protect from moisture,keep the vial protected from light

Stock: -20 °C

Stockage commentaire: The product is shipped as a lyophilized powder at room temperature. Upon receipt, store at -20°C, protect from moisture, and keep the vial protected from light.

Détail du antigène

Antigène: NGF 2.5S

Sujet: A fluorescent mouse NGF 2.5S for sensitive detection, live-cell imaging, and tracking of TrkA and p75NTR receptors and NGF internalization dynamics

Poids moléculaire: ~26.6 kDa (dimer)