IgG1 Fc (AA 100-330) (Active) Protéine

N° du produit ABIN7596113

Cette protéine Recombinant est exprimée dans HEK-293 Cells.

Aperçu rapide pour IgG1 Fc (AA 100-330) (Active) Protéine (ABIN7596113)

Antigène: IgG1 Fc

Type de proteíne: Recombinant

Activité biologique: Active

Origine: Humain

Source: HEK-293 Cells

Application: Activity Assay (AcA), SDS-PAGE (SDS)

Pureté: > 90% by SDS-PAGE

Détail du produit

Attributs du protein: AA 100-330

Séquence: PKSCDKTHTC PPCPAPELLG GPSVFLFPPK PKDTLMISRT PEVTCVVVDV SHEDPEVKFN WYVDGVEVHN AKTKPREEQY NSTYRVVSVL TVLHQDWLNG KEYKCKVSNK ALPAPIEKTI SKAKGQPREP QVYTLPPSRD ELTKNQVSLT CLVKGFYPSD IAVEWESNGQ PENNYKTTPP VLDSDGSFFL YSKLTVDKSR WQQGNVFSCS VMHEALHNHY TQKSLSLSPG K

niveau d'endotoxine: < 1 EU per 1ug of protein (determined by LAL method)

Biological Activity Comment: 1. Measured by its binding ability in a functional ELISA with Human Fc gamma RIIIA/CD16a (CAT# ATGP4102). The ED50 range ≤ 1.5 ug/ml., 2. Measured by its binding ability in a functional ELISA with Human Fc gamma RIIIA/CD16a (CAT# ATGP4116)., 3. Measured by its binding ability in a functional ELISA with Human Fc gamma RIIIA/CD16a (CAT# ATGP4098). The ED50 range ≤ 4 ug/ml.

Information d'application

Indications d'application: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 1 mg/mL

Stock: 4 °C,-20 °C,-80 °C

Stockage commentaire: Can be stored at +2°C to +8°C for 1 week. For long term storage, aliquot and store at -20°C to -80°C. Avoid repeated freezing and thawing cycles.

Détail du antigène

Antigène: IgG1 Fc

Sujet: Immunoglobulins are membrane-bound or secreted glycoproteins produced by B lymphocytes. IgG antibodies protect the body against the pathogens by agglutination and immobilization, complement activation, toxin neutralization, as well as the antibody-dependent cell-mediated cytotoxicity. IgG1 is most abundant in serum among the four IgG subclasses (IgG1, 2, 3 and 4) and binds to Fc receptors on phagocytic cells with high affinity. IgG comprise two identical light (L) and heavy (H) chains forming a 'Y-shaped' structure. It also is digested by pepsin proteolysis into two distinct fragments (Fab, Fc). Fc fragment is a homodimeric glycoprotein with one monomer consisting of two constant immunoglobulin domains (CH2, CH3) from the C-terminal half of one heavy chain. Through interaction with its ligands (FcγR, C1q and FcRn) it mediates various immune effector functions and increases the half-life of the antibody molecule. Recombinant IgG Fc is suggested to represent a potential anti-inflammatory drug for treatment of human autoimmune diseases. Recombinant human IgG1 Fc was expressed in HEK293 cell and purified by using conventional chromatography techniques.

Poids moléculaire: 25.9kDa (231aa)