PAR1 Protein (DYKDDDDK Tag,Strep Tag)

N° du produit ABIN7596916

Cette protéine Synthetic Nanodisc PAR1 est produite dans HEK-293 Cells.

Aperçu rapide pour PAR1 Protein (DYKDDDDK Tag,Strep Tag) (ABIN7596916)

Antigène: PAR1 (F2R) (Coagulation Factor II (thrombin) Receptor (F2R))

Type de proteíne: Synthetic Nanodisc

Origine: Humain

Source: HEK-293 Cells

Application: Immunogen (Imm), ELISA, Cryogenic electron microscopy (cryo-EM), Phage Display (PhD), Surface Plasmon Resonance (SPR)

Détail du produit

Purification/Conjugué: Cette PAR1 protéine est marqué à la DYKDDDDK Tag,Strep Tag.

Fonction: Human PAR1-Strep full length protein-synthetic nanodisc

Attributs du produit: Unlike other membrane scaffold protein (MSP) Nanodisc on the market, our synthetic Nanodisc can be prepared directly from the cells. The polymers used during this process have a dual function. It dissolves the cell membranes, like the detergent, and uses cellular phospholipids to form Nanodisc around the membrane proteins. The target protein embedded Nanodiscs can then be purified.

Information d'application

Indications d'application: Optimal working dilution should be determined by the investigator.

Commentaires:

Advantages:

• Highly purified membrane proteins
• High solubility in aqueous solutions
• High stability
• Proteins are in a native membrane environment and remain biologically active
• No detergent and can be used for cell-based assays
• No MSP backbone proteins

Limitations:

• Intolerant to acids and high concentrations of divalent metal ions

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: Please see Certificate of Analysis for specific instructions of reconstitution

Buffer: Lyophilized from nanodisc solubilization buffer (20 mM Tris-HCl, 150 mM NaCl, pH 8.0). Normally 5 % - 8 % trehalose is added as protectants before lyophilization.

Stock: -20 °C,-80 °C

Stockage commentaire: Store at -20°C to -80°C for 12 months in lyophilized form. After reconstitution, if not intended for use within a month, aliquot and store at -80°C (Avoid repeated freezing and thawing). Lyophilized proteins are shipped at ambient temperature.

Date de péremption: 12 months

Détail du antigène

Antigène: PAR1 (F2R) (Coagulation Factor II (thrombin) Receptor (F2R))

Autre désignation: PAR1

Sujet: CF2R, HTR, PAR-1, PAR1, TR
Coagulation factor II receptor is a 7-transmembrane receptor involved in the regulation of thrombotic response. Proteolytic cleavage leads to the activation of the receptor. F2R is a G-protein coupled receptor family member. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Aug 2015]

Poids moléculaire: The human full length PAR1-Strep protein has a MW of 47.4 kDa

UniProt: P25116

Pathways: Nuclear Receptor Transcription Pathway, Skeletal Muscle Fiber Development, Positive Regulation of Endopeptidase Activity, Protein targeting to Nucleus