SHKB1 protein (DYKDDDDK Tag,Strep Tag)

N° du produit ABIN7597077

Protéine Synthetic Nanodisc SHKB1 exprimée dans HEK-293 Cells.

Aperçu rapide pour SHKB1 protein (DYKDDDDK Tag,Strep Tag) (ABIN7597077)

Antigène: SHKB1

Type de proteíne: Synthetic Nanodisc

Origine: Humain

Source: HEK-293 Cells

Application: Cryogenic electron microscopy (cryo-EM), ELISA, Immunogen (Imm), Phage Display (PhD), Surface Plasmon Resonance (SPR)

Détail du produit

Purification/Conjugué: DYKDDDDK Tag,Strep Tag

Fonction: Human SHKB1-Strep full length protein-synthetic nanodisc

Attributs du produit: Unlike other membrane scaffold protein (MSP) Nanodisc on the market, our synthetic Nanodisc can be prepared directly from the cells. The polymers used during this process have a dual function. It dissolves the cell membranes, like the detergent, and uses cellular phospholipids to form Nanodisc around the membrane proteins. The target protein embedded Nanodiscs can then be purified.

Information d'application

Indications d'application: Optimal working dilution should be determined by the investigator.

Commentaires:

Advantages:

• Highly purified membrane proteins
• High solubility in aqueous solutions
• High stability
• Proteins are in a native membrane environment and remain biologically active
• No detergent and can be used for cell-based assays
• No MSP backbone proteins

Limitations:

• Intolerant to acids and high concentrations of divalent metal ions

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: Please see Certificate of Analysis for specific instructions of reconstitution

Buffer: Lyophilized from nanodisc solubilization buffer (20 mM Tris-HCl, 150 mM NaCl, pH 8.0). Normally 5 % - 8 % trehalose is added as protectants before lyophilization.

Stock: -20 °C,-80 °C

Stockage commentaire: Store at -20°C to -80°C for 12 months in lyophilized form. After reconstitution, if not intended for use within a month, aliquot and store at -80°C (Avoid repeated freezing and thawing). Lyophilized proteins are shipped at ambient temperature.

Date de péremption: 12 months

Détail du antigène

Antigène: SHKB1

Sujet: PP203, Sb1
Inhibits CBL-SH3KBP1 complex mediated down-regulation of EGFR signaling by sequestration of SH3KBP1. Binds to SH3KBP1 and prevents its interaction with CBL and inhibits translocation of SH3KBP1 to EGFR containing vesicles upon EGF stimulation.[UniProtKB/Swiss-Prot Function]

Poids moléculaire: The human full length SHKB1-Strep protein has a MW of 76.3 kDa

UniProt: Q8TBC3