HFM1 Protein (AA 5325-5925) (His-MBP)

N° du produit ABIN7668895

Cette protéine Recombinant HFM1 est produite dans Escherichia coli (E. coli).

Aperçu rapide pour HFM1 Protein (AA 5325-5925) (His-MBP) (ABIN7668895)

Antigène: HFM1 (HFM1, ATP-Dependent DNA Helicase Homolog (HFM1))

Type de proteíne: Recombinant

Activité biologique: Inactive

Origine: SARS Coronavirus-2 (SARS-CoV-2)

Source: Escherichia coli (E. coli)

Pureté: > 80 %

Détail du produit

Attributs du protein: AA 5325-5925

Purification/Conjugué: Cette HFM1 protéine est marqué à la His-MBP.

Fonction: Recombinant SARS-CoV-2 Helicase Protein (His Tag)

Séquence: Ala5325-Gln5925

Purification: > 80 % as determined by reducing SDS-PAGE.

Stérilité: 0.2 μm filtered

niveau d'endotoxine: < 1.0 EU per μg of the protein as determined by the LAL method.

Information d'application

Restrictions: For Research Use only

Stockage

Format: Liquid

Buffer: Supplied as a 0.2 μm filtered solution of PBS, pH 7.4.

Stock: -20 °C

Stockage commentaire: Store at < -20°C, stable for 6 months. Please minimize freeze-thaw cycles.

Détail du antigène

Antigène: HFM1 (HFM1, ATP-Dependent DNA Helicase Homolog (HFM1))

Autre désignation: Helicase

Sujet: The non-structural protein 13 (nsp13) of SARS-CoV 2 is a helicase that separates double-stranded RNA or DNA with a 5'-3' polarity, using the energy of nucleotide hydrolysis. A basic biochemical characterization of nsp13 demonstrated that it can unwind both doublestranded DNA and RNA in a 5'-3' direction, and it can hydrolyze all deoxyribonucleotide and ribonucleotide triphosphates. Helicases are motor proteins that utilize the energy derived from nucleotide hydrolysisto unwind double-stranded nucleic acids into two single-stranded nucleic acids. Initially, helicases were only thought to be molecular engines that unwind nucleic acids during replication, recombination, and DNA repair. Recent studies have shown that they are also involved in other biological processes, including displacement of proteins from nucleic acid, movement of Holliday junctions, chromatin remodeling, catalysis of nucleic acid conformational changes, several aspects of RNA metabolism, including transcription, mRNA splicing, mRNA export, translation, RNA stability and mitochondrial gene expression. Some human diseases, including Bloom's syndrome, Werner's syndrome, and Xeroderma Pigmentosum have been associated with defects in helicase function.,SARS-CoV 2 Helicase,SARS-CoV 2 nsp13

Poids moléculaire:

calculated_mw: 112.8 kDa

observed_mw: 120 kDa