LTA Protein (His tag)

N° du produit ABIN7669249

Cette protéine Recombinant LTA est produite dans Escherichia coli (E. coli).

Aperçu rapide pour LTA Protein (His tag) (ABIN7669249)

Antigène: LTA (Lymphotoxin-alpha (LTA))

Type de proteíne: Recombinant

Activité biologique: Active

Origine: Souris

Source: Escherichia coli (E. coli)

Application: Cell Culture (CC)

Pureté: > 98 %

Détail du produit

Purification/Conjugué: Cette LTA protéine est marqué à la His tag.

Fonction: Recombinant Mouse TNF beta protein(His Tag)

Séquence: Leu 34-Leu 202

Purification: > 98 % as determined by reducing SDS-PAGE.

Stérilité: 0.2 μm filtered

niveau d'endotoxine: < 0.1 EU per μg of the protein as determined by the LAL method.

Biological Activity Comment: Measure by its ability to induce cytotoxicity in L929 cells in the presence of vactinomycin D. The ED₅₀ for this effect is <30 ng/mL.

Information d'application

Indications d'application: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Buffer: Lyophilized from sterile PBS, pH 8.0.
Normally 5 % - 8 % trehalose, mannitol and 0.01 % Tween 80 are added as protectants before lyophilization.

Stock: 4 °C,-20 °C,-80 °C

Stockage commentaire: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Date de péremption: 12 months

Détail du antigène

Antigène: LTA (Lymphotoxin-alpha (LTA))

Autre désignation: TNF beta

Sujet: Tumor Necrosis Factor β (TNF-β) is a secreted protein belonging to the tumor necrosis factor family. TNF-β binds to TNFRSF1A/TNFR1, TNFRSF1B/TNFBR and TNFRSF14/HVEM in homotrimeric form, binds to TNFRSF3/LTBR in heterotrimeric form with LTB. TNF-β forms heterotrimers with lymphotoxin-beta, which anchors TNF-β to the cell surface. TNF-β mediates the inflammatory, immunostimulatory, and antiviral response, involves in the formation of second lymphoid organs during development, has a role in apoptosis. TNF-β is produced by lymphocytes and cytotoxic for a variety of tumor cells in vitro and in vivo.,IFL,IFL-1,IFN-λ3,INF-alpha,INF-l,INF-lambda

Poids moléculaire:

calculated_mw: 19.4 kDa

observed_mw: 11-17 kDa

UniProt: P09225

Pathways: Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process