NOV Protein (Fc Tag)

N° du produit ABIN7669350

Protéine Recombinant NOV exprimée dans Mammalian Cells.

Aperçu rapide pour NOV Protein (Fc Tag) (ABIN7669350)

Antigène: NOV (Nephroblastoma Overexpressed (NOV))

Type de proteíne: Recombinant

Origine: Souris

Source: Mammalian Cells

Application: Immunogen (Imm)

Pureté: > 90 % as determined by reducing SDS-PAGE.

Détail du produit

Purification/Conjugué: Cette NOV protéine est marqué à la Fc Tag.

Fonction: Recombinant Mouse NOV/CCN3 Protein(Fc Tag)

Séquence: Gln22-lle354

Purification: SDS-PAGE analysis of Mouse NOV/CCN3 proteins, 2 μg/lane of Recombinant Mouse NOV/CCN3 proteins was resolved with SDS-PAGE under reducing conditions, showing bands at 80 KD

Stérilité: 0.2 μm filtered

niveau d'endotoxine: < 1.0 EU/mg of the protein as determined by the LAL method

Biological Activity Comment: Not validated for activity

Information d'application

Indications d'application: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.5 mg/mL. Concentration is measured by UV-Vis.

Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with 5 % Trehalose and 5 % Mannitol.

Stock: 4 °C,-20 °C,-80 °C

Stockage commentaire: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Date de péremption: 12 months

Détail du antigène

Antigène: NOV (Nephroblastoma Overexpressed (NOV))

Autre désignation: NOV

Sujet: CCN,CCN family member,NovH,CCN family member 3,Nephroblastoma-overexpressed gene protein homolog,Protein NOV homolog,Nov,Ccn3,Protein NOV homolog, also known as Nephroblastoma-overexpressed gene protein homolog, NOV, and CCN3, is a putative ligand for integrin receptors, is tightly associated with the extracellular matrix, and mediates diverse cellular functions, including cell adhesion and proliferation. CCN3 has been shown to negatively regulate growth althoμgh it promotes migration in a cell type-specific manner. This secreted protein belongs to the CCN family, and its expression was observed in a broad variety of tissues from the early stage of development, and altered expression of CCN3 has been observed in a variety of tumors, including hepatocellular carcinomas, Wilm's tumors, Ewing's sarcomas, gliomas, rhabdomyosarcomas, and adrenocortical carcinomas. Mature CCN3 protein has five distinct modules and truncated protein variants with altered function are found in many cancers. CCN3 acts throμgh the core stem cell signaling pathways including Notch and Bone Morphogenic Protein, connecting CCN3 with the modulation of self-renewal and maturation of some cell lineages including hematopoietic, osteogenic, and chondrogenic. CCN3 may affect the extracellular environment of the niche for hematopoietic stem cells. CCN3 has emerged as a key player in stem cell regulation, hematopoiesis, and a crucial component within the bone marrow microenvironment.

Poids moléculaire:

Calculated MW: 61.5 kDa

Observed MW: 80 kDa

UniProt: Q64299

Pathways: Smooth Muscle Cell Migration, Growth Factor Binding