Phenylalanine Hydroxylase Protein (His tag)

N° du produit ABIN7669418

Cette protéine Recombinant Phenylalanine Hydroxylase est exprimée dans Baculovirus infected Insect Cells.

Aperçu rapide pour Phenylalanine Hydroxylase Protein (His tag) (ABIN7669418)

Antigène: Phenylalanine Hydroxylase

Type de proteíne: Recombinant

Activité biologique: Inactive

Origine: Humain

Source: Baculovirus infected Insect Cells

Pureté: > 70 %

Détail du produit

Purification/Conjugué: Cette Phenylalanine Hydroxylase protéine est marqué à la His tag.

Fonction: Recombinant Human PAH/PH Protein (415 Asn/Asp, His Tag)

Séquence: Met 1-Lys 452

Purification: > 70 % as determined by reducing SDS-PAGE.

Stérilité: 0.2 μm filtered

niveau d'endotoxine: < 1.0 EU per μg of the protein as determined by the LAL method.

Information d'application

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Buffer: Lyophilized from sterile 20 mM Tris, 500 mM NaCl, pH 8.0, 10 % glycerol
Normally 5 % - 8 % trehalose, mannitol and 0.01 % Tween 80 are added as protectants before lyophilization.

Stock: 4 °C,-20 °C,-80 °C

Stockage commentaire: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Date de péremption: 12 months

Détail du antigène

Antigène: Phenylalanine Hydroxylase

Autre désignation: PAH

Sujet: PAH (phenylalanine hydroxylase), also known as PH, belongs to the biopterin-dependent aromatic amino acid hydroxylase family. It contains 1 ACT domain, N-terminal region of PAH is thought to contain allosteric binding sites for phenylalanine and to constitute an "inhibitory" domain that regulates the activity of a catalytic domain in the C-terminal portion of the molecule. In humans, PAH is expressed both in the liver and the kidney, and there is some indication that it may be differentially regulated in these tissues. PAH catalyzes the hydroxylation of the aromatic side-chain of phenylalanine to generate tyrosine. It is one of three members of the pterin-dependent amino acid hydroxylases, a class of monooxygenase that uses tetrahydrobiopterin and a non-heme iron for catalysis. Defects in PAH are the cause of phenylketonuria (PKU). PKU is an autosomal recessive inborn error of phenylalanine metabolism, due to severe phenylalanine hydroxylase deficiency. It is characterized by blood concentrations of phenylalanine persistently above 1200 mumol.,PH,PKU,PKU1

Poids moléculaire:

calculated_mw: 54.0 kDa

observed_mw: 50 kDa

UniProt: P00439