TSPAN33 Protein (DYKDDDDK Tag)

N° du produit ABIN7853967

Cette protéine MNP Membrane Nanoparticle TSPAN33 est exprimée dans HEK-293 Cells.

Aperçu rapide pour TSPAN33 Protein (DYKDDDDK Tag) (ABIN7853967)

Antigène: TSPAN33 (Tetraspanin 33 (TSPAN33))

Type de proteíne: MNP Membrane Nanoparticle

Origine: Humain

Source: HEK-293 Cells

Détail du produit

Purification/Conjugué: Cette TSPAN33 protéine est marqué à la DYKDDDDK Tag.

Fonction: Human TSPAN33 full length protein-MNP

Attributs du produit: Plasma membrane-coated nanoparticles (MNPs) have been used in various applications, including delivery of therapeutic agents and induction of immune responses et al. Unlike the conventional strategies, MNPs directly leverage intact and natural functions of cell membranes, and show high biocompatibility, specificity, and low side effects. Our optimized MNPs platform for the full-length membrane protein production uses membrane coating technology and a HEK293 based expression platform. The high-purity plasma membrane-coated nanoparticles were produced by extrusion after membrane extraction from the host HEK293 cells containing the overexpressed target proteins.

Information d'application

Commentaires:

Advantages of MNP:

• High display density of target membrane proteins
• Native structure and orientation of transmembrane protein
• soluble in aqueous solutions for routine biochemical analysis
• Detergent-free purification process
• Strong immunogenicity
• Works for MPs that can´t be produced via VLPs and EXos

Limitations of MNP:

• Lack of accurate quantification of the target membrane proteins.
• Need to develop special SPR assayx.
• Cell membranes contain housekeeping proteins that can result in immune response dilution.
• Some membrane proteins can´t be enriched on membrane.

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Buffer: Lyophilized from PBS. Normally 5 % - 8 % trehalose is added as protectants before lyophilization.

Stock: -20 °C,-80 °C

Stockage commentaire: Store at -20°C to -80°C for 12 months in lyophilized form. After reconstitution, if not intended for use within a month, aliquot and store at -80°C (Avoid repeated freezing and thawing). Lyophilized proteins are shipped at ambient temperature.

Date de péremption: 12 months

Détail du antigène

Antigène: TSPAN33 (Tetraspanin 33 (TSPAN33))

Autre désignation: TSPAN33

Sujet: PEN, PEN., TSPAN-33
Plays an important role in normal erythropoiesis (By similarity). It has a role in the differentiation of erythroid progenitors (By similarity). Regulates maturation and trafficking of the transmembrane metalloprotease ADAM10 (PubMed:26686862). Negatively regulates ligand-induced Notch activity probably by regulating ADAM10 activity (PubMed:26686862).

Poids moléculaire: The human full length TSPAN33 protein has a MW of 31.5 kDa

UniProt: Q86UF1