EML4-ALK (Active) protein (GST tag)

N° du produit ABIN7856243

Protéine Recombinant EML4-ALK exprimée dans Baculovirus infected Insect Cells.

Aperçu rapide pour EML4-ALK (Active) protein (GST tag) (ABIN7856243)

Antigène: EML4-ALK

Type de proteíne: Recombinant

Activité biologique: Active

Origine: Humain

Source: Baculovirus infected Insect Cells

Application: Kinase Activity Assay (KAA)

Détail du produit

Purification/Conjugué: GST tag

Fonction: Recombinant EML4-ALK (Eex6Aex20), Active

Séquence: EML4 (1-222) - ALK (1058-end)

Attributs du produit: Recombinant human fusion protein EML4 (1-222a.a., exons1-6) - ALK(1058a.a.-end, exons20-29) was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag. The gene accession number of EML4 is NM_019063, and ALK is NM_004304.

Stérilité: Sterile

Information d'application

Indications d'application: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Stockage

Format: Liquid

Buffer: Supplied as sterile 50 mM Tris-HCl, pH 7.5, 50-300 mM NaCl, 10 mM glutathione, 0.1 mM EDTA, 0.25-1.0 mM DTT, 0-0.1 mM PMSF, 10-25 % glycerol

Agent conservateur: Dithiothreitol (DTT), PMSF

Précaution d'utilisation: This product contains Dithiothreitol (DTT): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: -80 °C

Stockage commentaire: Store product at -70°C. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles. Product shipped on dry ice.

Date de péremption: 12 months

Détail du antigène

Antigène: EML4-ALK

Sub Type: Fusionprotein

Sujet: ALK or CD246 is a receptor tyrosine kinase, which belongs to the insulin receptor superfamily which comprises an extracellular domain, a hydrophobic stretch corresponding to a single pass transmembrane region, and an intracellular kinase domain. CD246 plays an important role in the development of the brain and exerts its effects on specific neurons in the nervous system. ALK-positive neoplasms represent a distinct entity because the morphology of the tumors is often neither anaplastic nor large cell and the tumors should be referred to as ALK lymphomas. CD246 has been found to be rearranged, mutated, or amplified in a series of tumours including anaplastic large cell lymphomas, neuroblastoma, and non-small cell lung cancer. EML4-ALK mutations in lung cancer confer resistance to ALK inhibitors.

Poids moléculaire: ~118 kDa

NCBI Accession: NM_019063, NM_004304