CRISPR-Cas9 protein (His tag)

N° du produit ABIN7884339

Protéine Recombinant CRISPR-Cas9 exprimée dans Escherichia coli (E. coli).

Aperçu rapide pour CRISPR-Cas9 protein (His tag) (ABIN7884339)

Antigène: CRISPR-Cas9

Type de proteíne: Recombinant

Origine: Streptococcus pyogenes

Source: Escherichia coli (E. coli)

Application: Western Blotting (WB)

Pureté: >98 %

Détail du produit

Purification/Conjugué: His tag

Fonction: wtCas9

Séquence: MDKKYSIGLD IGTNSVGWAV ITDEYKVPSK KFKVLGNTDR HSIKKNLIGA LLFDSGETAE ATRLKRTARR RYTRRKNRIC YLQEIFSNEM AKVD DSFFHRLEES FLVEEDKKHE RHPIFGNIVD EVAYHEKYPT IYHLRKKLVD STDKADLRLI YLALAHMIKF RGHFLIEGDL NPDNSDVDKL FIQLVQ TYNQLFEENP INASGVDAKA ILSARLSKSR RLENLIAQLP GEKKNGLFGN LIALSLGLTP NFKSNFDLAE DAKLQLSKDT YDDDLDNLLA QIGD QYADLFLAAK NLSDAILLSD ILRVNTEITK APLSASMIKR YDEHHQDLTL LKALVRQQLP EKYKEIFFDQ SKNGYAGYID GGASQEEFYK FIKPILE KMDGTEELLV KLNREDLLRK QRTFDNGSIP HQIHLGELHA ILRRQEDFYP FLKDNREKIE KILTFRIPYY VGPLARGNSR FAWMTRKSEE TITPW NFEEVVDKGA SAQSFIERMT NFDKNLPNEK VLPKHSLLYE YFTVYNELTK VKYVTEGMRK PAFLSGEQKK AIVDLLFKTN RKVTVKQLKE DYFK KIECFDSVEI SGVEDRFNAS LGTYHDLLKI IKDKDFLDNE ENEDILEDIV LTLTLFEDRE MIEERLKTYA HLFDDKVMKQ LKRRRYTGWG RLSRKLI NGIRDKQSGK TILDFLKSDG FANRNFMQLI HDDSLTFKED IQKAQVSGQG DSLHEHIANL AGSPAIKKGI LQTVKVVDEL VKVMGRHKPE NIV IEMARENQTT QKGQKNSRER MKRIEEGIKE LGSQILKEHP VENTQLQNEK LYLYYLQNGR DMYVDQELDI NRLSDYDVDH IVPQSFLKDD SI DNKVLTRSDK NRGKSDNVPS EEVVKKMKNY WRQLLNAKLI TQRKFDNLTK AERGGLSELD KAGFIKRQLV ETRQITKHVA QILDSRMNTK Y DENDKLIREV KVITLKSKLV SDFRKDFQFY KVREINNYHH AHDAYLNAVV GTALIKKYPK LESEFVYGDY KVYDVRKMIA KSEQEIGKAT AKYFF YSNIMNFFKT EITLANGEIR KRPLIETNGE TGEIVWDKGR DFATVRKVLS MPQVNIVKKT EVQTGGFSKE SILPKRNSDK LIARKKDWDP KKYG GFDSPTVAYS VLVVAKVEKG KSKKLKSVKE LLGITIMERS SFEKNPIDFL EAKGYKEVKK DLIIKLPKYS LFELENGRKR MLASAGELQK GNELALP SKYVNFLYLA SHYEKLKGSP EDNEQKQLFV EQHKHYLDEI IEQISEFSKR VILADANLDK VLSAYNKHRD KPIREQAENI IHLFTLTNLG APAAFK YFDTTIDRKR YTSTKEVLDA TLIHQSITGL YETRIDLSQL GGD with polyhistid ine tag at the C-terminus

niveau d'endotoxine: <0.1 EU per 1 μg protein

Information d'application

Indications d'application: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: dissolve in sterile H2O

Buffer: PBS, pH 7.4

Stock: -20 °C

Stockage commentaire: -20°C

Détail du antigène

Antigène: CRISPR-Cas9

Autre désignation: Cas9

Sujet: The wtCas9 is a recombinant Streptococcus pyogenes Cas9 protein (wild type form) purified from E. coli. Cas9 protein can form a very stable ribonucleoprotein (RNP) complex with the guide RNA (gRNA). The RNP complex recognizes the target site by matching gRNA with the genomic DNA sequence and leads to DNA breaks within 3 bases from the NGG PAM (Protospacer Adjacent Motif). With the CRISPR/Cas9 system, researchers can valid gene editing assay in cells or other kinds of animals.

Poids moléculaire: 158kDa

UniProt: Q99ZW2