CDK6/CyclinD3 (Complex) Protéine

N° du produit ABIN7938700

Protéine Recombinant CDK6/CyclinD3 exprimée dans Baculovirus infected Insect Cells.

Aperçu rapide pour CDK6/CyclinD3 (Complex) Protéine (ABIN7938700)

Antigène: CDK6/CyclinD3

Type de proteíne: Recombinant

Origine: Humain

Source: Baculovirus infected Insect Cells

Application: Functional Studies (Func), Activity Assay (AcA), Screening Assay (ScA)

Détail du produit

Attributs du protein: Complex

Fonction: Recombinant CDK6 / Cyclin D3 Complex

Information d'application

Indications d'application: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Stockage

Format: Liquid

Buffer: Recombinant CDK6 / Cyclin D3 Complex is supplied in 25 mM HEPES pH 7.5, 300 mM NaCl, 20 % glycerol, 0.04 % Triton X-100, 0.5 mM TCEP.

Conseil sur la manipulation: Avoid repeated freeze/thaw cycles,keep on ice when not in storage

Stock: -80 °C

Stockage commentaire: Recombinant proteins in solution are temperature sensitive and must be stored at -80°C to prevent degradation. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.

Détail du antigène

Antigène: CDK6/CyclinD3

Autre désignation: CDK6 & Cyclin D3

Sujet:

Protein Background: CDK6 is a serine/threonine-protein kinase involved in the control of the cell cycle and differentiation, promotes G1/S transition. Phosphorylates pRB/RB1 and NPM1. Interacts with D-type G1 cyclins during interphase at G1 to form a pRB/RB1 kinase and controls the entrance into the cell cycle. Involved in initiation and maintenance of cell cycle exit during cell differentiation, prevents cell proliferation and negatively regulates cell differentiation but is required for the proliferation of specific cell types (e.g. erythroid and hematopoietic cells). Essential for cell proliferation within the dentate gyrus of the hippocampus and the subventricular zone of the lateral ventricles. Required during thymocyte development Promotes the production of newborn neurons, probably by modulating G1 length. Promotes, at least in astrocytes, changes in patterns of gene expression, changes in the actin cytoskeleton including loss of stress fibers, and enhanced motility during cell differentiation. Prevents myeloid differentiation by interfering with RUNX1 and reducing its transcription transactivation activity but promotes proliferation of normal myeloid progenitors. Delays senescence. Promotes the proliferation of beta-cells in pancreatic islets of Langerhans. May play a role in the centrosome organization during the cell cycle phases.

Short Description: Recombinant CDK6 / Cyclin D3 Complex that includes full length human CDK6 protein (accession number NP_001250.1) with an N-terminal His tag and full-length human Cyclin D3 protein (accession number NP_001751.1) with an C-terminal FLAG tag was expressed in Hi5 cells. The molecular weights of CDK6 and Cyclin D3 are 40.03 kDa and 34.21 kDa, respectively.