CHD8 Protéine

N° du produit ABIN7938718

Protéine Recombinant CHD8 exprimée dans Escherichia coli (E. coli).

Aperçu rapide pour CHD8 Protéine (ABIN7938718)

Antigène: CHD8 (Chromodomain Helicase DNA Binding Protein 8 (CHD8))

Type de proteíne: Recombinant

Origine: Humain

Source: Escherichia coli (E. coli)

Application: Activity Assay (AcA), Functional Studies (Func), Screening Assay (ScA)

Détail du produit

Fonction: Recombinant CHD8 protein

Information d'application

Indications d'application: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Stockage

Format: Liquid

Buffer: Recombinant CHD8 protein is supplied in 25 mM HEPES pH 7.5, 300 mM NaCl, 20 % glycerol, 0.04 % Triton X-100, 0.5 mM TCEP.

Conseil sur la manipulation: Avoid repeated freeze/thaw cycles,keep on ice when not in storage

Stock: -80 °C

Stockage commentaire: Recombinant proteins in solution are temperature sensitive and must be stored at -80°C to prevent degradation. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.

Détail du antigène

Antigène: CHD8 (Chromodomain Helicase DNA Binding Protein 8 (CHD8))

Autre désignation: CHD8

Sujet:

Protein Background: CHD8 is a DNA helicase, that acts as a chromatin remodeling factor and regulates transcription. Acts as a transcription repressor by remodeling chromatin structure and recruiting histone H1 to target genes. Suppresses p53/TP53-mediated apoptosis by recruiting histone H1 and preventing p53/TP53 transactivation activity. Acts as a negative regulator of Wnt signaling pathway by regulating beta-catenin (CTNNB1) activity. Negatively regulates CTNNB1-targeted gene expression by being recruited specifically to the promoter regions of several CTNNB1 responsive genes. Involved in both enhancer blocking and epigenetic remodeling at chromatin boundary via its interaction with CTCF. Acts as a suppressor of STAT3 activity by suppressing the LIF-induced STAT3 transcriptional activity. Also acts as a transcription activator via its interaction with ZNF143 by participating in efficient U6 RNA polymerase III transcription. Regulates alternative splicing of a core group of genes involved in neuronal differentiation, cell cycle and DNA repair. Enables H3K36me3-coupled transcription elongation and co-transcriptional RNA processing likely via interaction with HNRNPL.

Short Description: Recombinant CHD8 protein was expressed in a baculovirus expression system as the full-length protein (accession number NP_065971.2) with an N-terminal FLAG tag. The molecular weight of CHD8 is 263.6 kDa.

Aliases: Chromodomain helicase DNA binding protein 8, KIAA1564, HELSNF1, DUPLIN, IDDAM

Pathways: Chromatin Binding