eEF1A1 Protéine

N° du produit ABIN7938816

Cette protéine Recombinant eEF1A1 est produite dans Escherichia coli (E. coli).

Aperçu rapide pour eEF1A1 Protéine (ABIN7938816)

Antigène: eEF1A1 (EEF1A1) (Eukaryotic Translation Elongation Factor 1 alpha 1 (EEF1A1))

Type de proteíne: Recombinant

Origine: Humain

Source: Escherichia coli (E. coli)

Application: Activity Assay (AcA), Functional Studies (Func), Screening Assay (ScA)

Détail du produit

Fonction: Recombinant EEF1A1 protein

Attributs du produit: EEF1A1 (Eukaryotic Translation Elongation Factor 1 Alpha 1), also called as LENG7, CTCL Tumor Antigen or CCS3, is an isoform of the alpha subunit of the elongation factor-1 complex, which is responsible for the enzymatic delivery of aminoacyl tRNAs to the ribosome. This isoform (alpha 1) is expressed in brain, placenta, lung, liver, kidney, and pancreas. EEF1A1 is identified as an autoantigen in 66 % of patients with Felty syndrome. EEF1A1 can promote the GTP-dependent binding of aminoacyl-tRNA to the A-site of ribosomes during protein biosynthesis. With PARP1 and TXK, it can form a complex that acts as a T helper 1 (Th1) cell-specific transcription factor and binds the promoter of IFN-gamma to directly regulate its transcription, and is thus involved importantly in Th1 cytokine production. It may also mediate accelerated synthesis of free thiol-containing proteins in response to HC-induced oxidative stress by homocysteine (HC).

Information d'application

Indications d'application: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Stockage

Format: Liquid

Buffer: Recombinant EEF1A1 protein is supplied in 25 mM Tris-HCl pH 7.4, 500 mM NaCl, 10 % glycerol, 0.5 mM TCEP.

Conseil sur la manipulation: Avoid repeated freeze/thaw cycles,keep on ice when not in storage

Stock: -80 °C

Stockage commentaire: Recombinant proteins in solution are temperature sensitive and must be stored at -80°C to prevent degradation. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.

Détail du antigène

Antigène: eEF1A1 (EEF1A1) (Eukaryotic Translation Elongation Factor 1 alpha 1 (EEF1A1))

Autre désignation: EEF1A1

Sujet:

Protein Background: EEF1A1 (Eukaryotic Translation Elongation Factor 1 Alpha 1), also called as LENG7, CTCL Tumor Antigen or CCS3, is an isoform of the alpha subunit of the elongation factor-1 complex, which is responsible for the enzymatic delivery of aminoacyl tRNAs to the ribosome. This isoform (alpha 1) is expressed in brain, placenta, lung, liver, kidney, and pancreas. EEF1A1 is identified as an autoantigen in 66 % of patients with Felty syndrome. EEF1A1 can promote the GTP-dependent binding of aminoacyl-tRNA to the A-site of ribosomes during protein biosynthesis. With PARP1 and TXK, it can form a complex that acts as a T helper 1 (Th1) cell-specific transcription factor and binds the promoter of IFN-gamma to directly regulate its transcription, and is thus involved importantly in Th1 cytokine production. It may also mediate accelerated synthesis of free thiol-containing proteins in response to HC-induced oxidative stress by homocysteine (HC).

Short Description: Recombinant EEF1A1 protein was expressed in E.coli as the full length protein (accession number NP_001393.1) with an N-terminal 6xHis Tag. The molecular weight of the protein is 53.7 kDa.

Aliases: Eukaryotic Translation Elongation Factor 1 Alpha 1