EIF4A1 Protéine

N° du produit ABIN7938830

Protéine Recombinant EIF4A1 exprimée dans Escherichia coli (E. coli).

Aperçu rapide pour EIF4A1 Protéine (ABIN7938830)

Antigène: EIF4A1 (Eukaryotic Translation Initiation Factor 4A1 (EIF4A1))

Type de proteíne: Recombinant

Origine: Humain

Source: Escherichia coli (E. coli)

Application: Activity Assay (AcA), Functional Studies (Func), Screening Assay (ScA)

Détail du produit

Fonction: Recombinant EIF4A1 protein

Attributs du produit: EIF4A1 (Eukaryotic Translation Initiation Factor 4A, Isoform 1), also called as EIF4A or DDX2A, is an ATP-dependent RNA helicase which is a subunit of the eIF4F complex involved in cap recognition. It is required for mRNA binding to ribosome. In the current model of translation initiation, eIF4A unwinds RNA secondary structures in the 5'UTR of mRNAs which is necessary to allow efficient binding of the small ribosomal subunit, and subsequent scanning for the initiator codon. This protein is involved in the binding of mRNA to 40S subunit of ribosome, ubiquitous with high expression in skeletal muscle and ovary. The helicase activity of EIF4A1 and function in translation are inhibited by interaction with PDCD4.

Information d'application

Indications d'application: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Stockage

Format: Liquid

Buffer: Recombinant EIF4A1 protein is supplied in 25 mM Tris-HCl pH 8.0, 300 mM NaCl, 20 % glycerol, and 0.5 mM TCEP.

Conseil sur la manipulation: Avoid repeated freeze/thaw cycles,keep on ice when not in storage

Stock: -80 °C

Stockage commentaire: Recombinant proteins in solution are temperature sensitive and must be stored at -80°C to prevent degradation. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.

Détail du antigène

Antigène: EIF4A1 (Eukaryotic Translation Initiation Factor 4A1 (EIF4A1))

Autre désignation: EIF4A1

Sujet:

Protein Background: EIF4A1 (Eukaryotic Translation Initiation Factor 4A, Isoform 1), also called as EIF4A or DDX2A, is a ATP dependent RNA helicase which is a subunit of the eIF4F complex involved in cap recognition. It is required for mRNA binding to ribosome. In the current model of translation initiation, eIF4A unwinds RNA secondary structures in the 5'-UTR of mRNAs which is necessary to allow efficient binding of the small ribosomal subunit, and subsequent scanning for the initiator codon. This protein is involved in the binding of mRNA to 40S subunit of ribosome,ubiquitous with high expression in skeletal muscle and ovary. The helicase activity of EIF4A1 and function in translation are inhibited by interaction with PDCD4.

Short Description: Recombinant EIF4A1 protein was expressed in E.coli as the full length protein (accession number NP_001407.1) with an N-terminal 6xHis Tag. The molecular weight of the protein is 49.7 kDa.

Aliases: Eukaryotic Translation Initiation Factor 4A1, DDX2A