K-RAS Protein (AA 1-186, G12V)

N° du produit ABIN7938881

Cette protéine Recombinant K-RAS est produite dans Escherichia coli (E. coli).

Aperçu rapide pour K-RAS Protein (AA 1-186, G12V) (ABIN7938881)

Antigène: K-RAS (KRAS) (GTPase Kras (KRAS))

Type de proteíne: Recombinant

Origine: Humain

Source: Escherichia coli (E. coli)

Application: Screening Assay (ScA), Functional Studies (Func), Activity Assay (AcA)

Détail du produit

Attributs du protein: AA 1-186, G12V

Fonction: Recombinant KRAS (1-186, G12V) protein

Information d'application

Indications d'application: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Stockage

Format: Liquid

Buffer: Recombinant KRAS (1-186, G12V) protein is supplied in 25 mM Tris 8.0, 300 mM NaCl, 20 % glycerol, 0.5 mM TCEP.

Conseil sur la manipulation: Avoid repeated freeze/thaw cycles,keep on ice when not in storage

Stock: -80 °C

Stockage commentaire: Recombinant proteins in solution are temperature sensitive and must be stored at -80°C to prevent degradation. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.

Détail du antigène

Antigène: K-RAS (KRAS) (GTPase Kras (KRAS))

Autre désignation: KRAS

Sujet:

Protein Background: KRAS (GTPase KRas) protein is a GDP/GTP binding protein, and the more important genes of the same family include HRAS and NRAS. KRAS is activated when combined with GTP and closed when combined with GDP. KRAS can be transiently activated by growth factors or tyrosine kinases (such as EGFR). The activated KRAS can activate downstream signals such as PI3K-AKT-mTOR that control cell production pathway, and the RAS-RAF-MEK-ERK signaling pathway that controls cell proliferation. Mutant KRAS will continue to be activated even without activation of kinases such as EGFR, resulting in continued cell proliferation and eventually canceration. KRAS mutations are found in a variety of tumors, the most common ones include lung cancer and pancreatic cancer.

Short Description: Recombinant KRAS (1-186, G12V) protein that includes amino acids 1-186 of human KRAS protein (accession number NP_203524.1) was expressed in E. coli cells with a G12V mutation and a C-terminal 6xHis tag. The molecular weight of the protein is 22.36 kDa.

Aliases: GTPase KRas, KRAS1, KRAS2, Kirsten Rat Sarsoma Viral Oncogene Homolog, C-Ki-Ras, KRAS3B, K-Ras P21 Protein