Histone H3.3 Protein (G34V, Nucleosome)

N° du produit ABIN7938947

Cette protéine Recombinant Histone H3.3 est produite dans Escherichia coli (E. coli).

Aperçu rapide pour Histone H3.3 Protein (G34V, Nucleosome) (ABIN7938947)

Antigène: Histone H3.3 (H3F3A) (H3 Histone, Family 3A (H3F3A))

Type de proteíne: Recombinant

Origine: Humain

Source: Escherichia coli (E. coli)

Application: Screening Assay (ScA), Enzyme Kinetics (EK)

Pureté: >95 % pure by SDS-PAGE

Détail du produit

Attributs du protein: G34V, Nucleosome

Fonction: Recombinant Polynucleosomes H3.3 (G34V)

Attributs du produit: Recombinant Human Polynucleosomes (G34V) protein contains a Gly34Val point mutation. Nucleosomes comprise the smallest subunit of chromatin, they consist of 147 bp of DNA wrapped around an octamer of core histone proteins (H2A, H2B, H3 and H4). Therefore, nucleosomes are more physiologically relevant substrates than histones and histone-derived peptides for use in in vitro studies. More importantly, some histone methyltransferases are significantly more active, as well as specific, when using nucleosomal substrates in HMT assays, such as DOT1L and NSD family enzymes. Nucleosomes are also widely used in histone methyltransferase screening assays to identify small molecular inhibitors for drug discovery.

Information d'application

Indications d'application: Recombinant Polynucleosomes H3.3 (G34V) are suitable as substrate for use in the study of enzyme kinetics, inhibitor screening, and selectivity profiling.

Restrictions: For Research Use only

Stockage

Format: Liquid

Buffer: Human Recombinant Polynucleosomes H3.3 (G34V) (20 μg protein+ 24 μg DNA) are supplied in 10 mM Tris pH 8.0, 1 mM EDTA, 2 mM DTT, 20 % glycerol.

Agent conservateur: Dithiothreitol (DTT)

Précaution d'utilisation: This product contains Dithiothreitol (DTT): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Avoid repeated freeze/thaw cycles,keep on ice when not in storage

Stock: -80 °C

Stockage commentaire: Recombinant proteins in solution are temperature sensitive and must be stored at -80°C to prevent degradation. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.

Détail du antigène

Antigène: Histone H3.3 (H3F3A) (H3 Histone, Family 3A (H3F3A))

Autre désignation: Histone H3.3

Sujet:

Protein Background: In vivo, the nucleosome is the basic structural unit of chromatin. It is comprised of about 146 bp of DNA wrapped around a core of eight histones of four different types: H2A, H2B, H3 and H4. Histones are subject to posttranslational modifications, such as methylation, acetylation, phosphorylation and mono-ubiquitination. Histone modifications influence multiple chromatin-templated processes such as gene transcription, DNA repair and recombination. Besides the ""major"" histones, there are some histone variants in specific regions of chromatin or in specific cell types. Histone variants are involved in multiple processes including chromosome segregation, DNA repair, transcriptional regulation and mRNA processing. Histone H3.3 point mutations (K27 and G34) are found in 1/3 of pediatric glioblastomas. Up to 78 % of diffuse intrinsic pontine gliomas (DIPGs) carry K27M and 36 % of non-brainstem gliomas carry either K27M or G34R/V mutations. High- frequency mutation of histone H3 to K36M is observed in chondroblastomas, as is the mutation G34W/L in giant cell tumors of bone, diseases of adolescents and young adults. Histone H3.3 mutations drive pediatric glioblastoma through upregulation of MYCN. Nucleosomes are more physiologically relevant substrates than histones and histone-derived peptides for in vitro studies. More importantly, some histone methyltransferases are significantly more active, as well as specific, when using nucleosomal substrates in HMT assays, such as DOT1L and NSD family enzymes. Nucleosomes are also widely used in histone methyltransferase screening assays to identify small molecular inhibitors for drug discovery.

Short Description: Recombinant Polynucleosomes H3.3 (G34V), Human, consist of 5000 bp of DNA (plasmid pG5E4) and two molecules each of histones H2A that includes amino acids 1-130 (end) (accession number NM_003512), H2B that includes amino acids 1-126 (end) (accession number NM_003518), H3.3 that includes amino acids 1-136 (end) (accession number NM_005324) with a point mutation Gly34Val, and H4 that includes amino acids 1-103 (end) (accession number NM_003548). Plasmid pG5E4 contains 9 of 5S rDNA nucleosome positioning sequences of L. variegatus, 5 of GAL4 binding sites and E4 promoter. Every 5S rDNA can wrap one histone octamer to form a nucleosome. 5 of GAL4 binding sites and E4 promoter can wrap histone octamers to form dinucleosomes. A pG5E4 plasmid can warp histone octamers to form 11 nucleosomes. The recombinant protein is >95 % pure by SDS-PAGE.

Aliases: Histone H3.3, Histone H3, H3, G34V