MBD2 Protein (Methyl-CpG-binding domain)

N° du produit ABIN7939274

Protéine Recombinant MBD2 exprimée dans Escherichia coli (E. coli).

Aperçu rapide pour MBD2 Protein (Methyl-CpG-binding domain) (ABIN7939274)

Antigène: MBD2 (Methyl-CpG Binding Domain Protein 2 (MBD2))

Type de proteíne: Recombinant

Origine: Humain

Source: Escherichia coli (E. coli)

Application: Activity Assay (AcA), Functional Studies (Func), Screening Assay (ScA)

Détail du produit

Attributs du protein: Methyl-CpG-binding domain

Fonction: Recombinant MBD2-MBD protein

Information d'application

Indications d'application: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Stockage

Format: Liquid

Buffer: Recombinant MBD2-MBD protein is supplied in 25 mM Tris 8.0, 300 mM NaCl, 20 % glycerol, 0.5 mM TCEP.

Conseil sur la manipulation: Avoid repeated freeze/thaw cycles,keep on ice when not in storage

Stock: -80 °C

Stockage commentaire: Recombinant proteins in solution are temperature sensitive and must be stored at -80°C to prevent degradation. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.

Détail du antigène

Antigène: MBD2 (Methyl-CpG Binding Domain Protein 2 (MBD2))

Autre désignation: MBD2

Sujet:

Protein Background: MBD2 (Methyl-CpG-binding domain protein 2)is a transcriptional repressor that Binds CpG islands in promoters where the DNA is methylated at position 5 of cytosine within CpG dinucleotides. Also binds hemimethylated DNA as well and recruits histone deacetylases and DNA methyltransferases to chromatin. Acts as a component of the histone deacetylase NuRD complex which participates in the remodeling of chromatin. Functions as a scaffold protein, targeting GATAD2A and GATAD2B to chromatin to promote repression. May enhance the activation of some unmethylated cAMP-responsive promoters.

Short Description: Recombinant MBD2-MBD protein that includes amino acids 140-219 of human MBD2 protein (accession number NP_003918.1) was expressed in E. coli cells with an N-terminal 6xHis tag. The molecular weight of the protein is 11.19 kDa.

Aliases: Methyl-CpG -binding domain protein 2, CXXC3, PCM1, Protein Containing Methyl-CpG Binding Domain 1

Pathways: Chromatin Binding