NEK2 Protéine

N° du produit ABIN7939373

Cette protéine Recombinant NEK2 est produite dans Baculovirus infected Insect Cells.

Aperçu rapide pour NEK2 Protéine (ABIN7939373)

Antigène: NEK2 (NIMA (Never in Mitosis Gene A)-Related Kinase 2 (NEK2))

Type de proteíne: Recombinant

Origine: Humain

Source: Baculovirus infected Insect Cells

Application: Functional Studies (Func), Activity Assay (AcA), Screening Assay (ScA)

Détail du produit

Fonction: Recombinant NEK2 protein

Information d'application

Indications d'application: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Stockage

Format: Liquid

Buffer: Recombinant NEK2 protein is supplied in 25 mM HEPES-NaOH pH 7.5, 300 mM NaCl, 10 % glycerol, 0.04 % Triton X-100, 0.5 mM TCEP.

Conseil sur la manipulation: Avoid repeated freeze/thaw cycles,keep on ice when not in storage

Stock: -80 °C

Stockage commentaire: Recombinant proteins in solution are temperature sensitive and must be stored at -80°C to prevent degradation. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.

Détail du antigène

Antigène: NEK2 (NIMA (Never in Mitosis Gene A)-Related Kinase 2 (NEK2))

Autre désignation: NEK2

Sujet:

Protein Background: NEK2 (NIMA Related Kinase 2) is a protein kinase which is involved in the control of centrosome separation and bipolar spindle formation in mitotic cells and chromatin condensation in meiotic cells. NEK2 regulates centrosome separation (essential for the formation of bipolar spindles and high-fidelity chromosome separation) by phosphorylating centrosomal proteins such as CROCC, CEP250 and NINL, resulting in their displacement from the centrosomes and regulates kinetochore microtubule attachment stability in mitosis via phosphorylation of NDC80. NEK2 is involved in regulation of mitotic checkpoint protein complex via phosphorylation of CDC20 and MAD2L1 and plays an active role in chromatin condensation during the first meiotic division through phosphorylation of HMGA2. It is also essential for localization of MAD2L1 to kinetochore and MAPK1 and NPM1 to the centrosome. NEK2-mediated phosphorylation of CEP68 promotes CEP68 dissociation from the centrosome and its degradation at the onset of mitosis.

Short Description: Recombinant NEK2 protein was expressed in baculovirus expression system as the full-length protein (accession number NP_002488.1) with a N-terminal FLAG tag. The molecular weight of the protein is 53 kDa.

Aliases: NIMA Related Kinase 2

Pathways: M Phase