RNF4 Protéine

N° du produit ABIN7939518

Cette protéine Recombinant RNF4 est exprimée dans Escherichia coli (E. coli).

Aperçu rapide pour RNF4 Protéine (ABIN7939518)

Antigène: RNF4 (Ring Finger Protein 4 (RNF4))

Type de proteíne: Recombinant

Origine: Humain

Source: Escherichia coli (E. coli)

Application: Activity Assay (AcA), Functional Studies (Func), Screening Assay (ScA)

Détail du produit

Fonction: Recombinant RNF4 protein

Information d'application

Indications d'application: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Stockage

Format: Liquid

Buffer: Recombinant RNF4 protein is supplied in 25 mM Tris 8.0, 300 mM NaCl, 20 % glycerol, 0.5 mM TCEP.

Conseil sur la manipulation: Avoid repeated freeze/thaw cycles,keep on ice when not in storage

Stock: -80 °C

Stockage commentaire: Recombinant proteins in solution are temperature sensitive and must be stored at -80°C to prevent degradation. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.

Détail du antigène

Antigène: RNF4 (Ring Finger Protein 4 (RNF4))

Autre désignation: RNF4

Sujet:

Protein Background: RNF4 (E3 ubiquitin-protein ligase RNF4) also known as RES4-26, SLX5 and SNURF. RNF4 is a E3 ubiquitin-protein ligase which binds polysumoylated chains covalently attached to proteins and mediates 'Lys-6'- , 'Lys-11'-, 'Lys-48'- and 'Lys-63'-linked polyubiquitination of those substrates and their subsequent targeting to the proteasome for degradation. Regulates the degradation of several proteins including PML and the transcriptional activator PEA3. Catalyzes ubiquitination of sumoylated PARP1 in response to PARP1 trapping to chromatin, leading to PARP1 removal from chromatin by VCP/p97. Alternatively, it may also bind DNA/nucleosomes and have a more direct role in the regulation of transcription for instance enhancing basal transcription and steroid receptor-mediated transcriptional activation.

Short Description: Recombinant RNF4 protein that includes full length of human RNF4 protein (accession number NP_001171938.1) was expressed in E. coli and contains an N-terminal His tag with a molecular weight of 23.48 kDa. The purity of the protein is ≥ 95 % by SDS-PAGE.

Aliases: E3 ubiquitin-protein ligase RNF4, RES4-26, SLX5, SNURF

Pathways: Intracellular Steroid Hormone Receptor Signaling Pathway