YTHDC1 Protein (full length)

N° du produit ABIN7939762

Protéine Recombinant YTHDC1 exprimée dans Baculovirus infected Insect Cells.

Aperçu rapide pour YTHDC1 Protein (full length) (ABIN7939762)

Antigène: YTHDC1 (YTH Domain Containing 1 (YTHDC1))

Type de proteíne: Recombinant

Origine: Humain

Source: Baculovirus infected Insect Cells

Application: Screening Assay (ScA), Activity Assay (AcA), Functional Studies (Func)

Détail du produit

Attributs du protein: full length

Fonction: Recombinant YTHDC1 protein

Attributs du produit: Recombinant YTHDC1 protein was expressed in a baculovirus expression system as the full-length protein (accession number NP_001026902.1) with an N-terminal Flag tag. The molecular weight of the protein is 85.6 kDa. It is suitable for use in binding assays, inhibitor screening, and selectivity profiling.

Information d'application

Indications d'application: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Stockage

Format: Liquid

Buffer: Recombinant YTHDC1 protein is supplied in 25 mM HEPES-NaOH pH 7.5, 300 mM NaCl, 10 % glycerol, 0.04 % Triton X-100, 0.5 mM TCEP.

Conseil sur la manipulation: Avoid repeated freeze/thaw cycles,keep on ice when not in storage

Stock: -80 °C

Stockage commentaire: Recombinant proteins in solution are temperature sensitive and must be stored at -80°C to prevent degradation. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.

Détail du antigène

Antigène: YTHDC1 (YTH Domain Containing 1 (YTHDC1))

Autre désignation: YTHDC1

Sujet:

Protein Background: YTHDC1 (YTH Domain Containing 1), is a regulator of alternative splicing that specifically recognizes and binds N6-methyladenosine (m6A)-containing RNAs. M6A is a modification present at internal sites of mRNAs and some non-coding RNAs and plays a role in the efficiency of mRNA splicing, processing and stability. YTHDC1 acts as a key regulator of exon-inclusion or exon-skipping during alternative splicing via interaction with mRNA splicing factors SRSF3 and SRSF10. It specifically binds m6A-containing mRNAs and promotes recruitment of SRSF3 to its mRNA-binding elements adjacent to m6A sites, leading to exon-inclusion during alternative splicing. In contrast, interaction with SRSF3 prevents interaction with SRSF10, a splicing factor that promotes exon skipping: this prevents SRSF10 from binding to its mRNA-binding sites close to m6A-containing regions, leading to inhibit exon skipping during alternative splicing. YTHDC1 may also regulate alternative splice site selection.

Short Description: Recombinant YTHDC1 protein was expressed in a baculovirus expression system as the full length protein (accession number NP_001026902.1) with an N-terminal FLAG tag. The molecular weight of the protein is 85.6 kDa.

Aliases: YTH Domain Containing 1, Splicing Factor YT521

Poids moléculaire: 85.6 kDa

NCBI Accession: NP_001026902