PI3K p85/p55 Protein (Complex)

N° du produit ABIN7939821

Cette protéine Recombinant PI3K p85/p55 est produite dans Baculovirus infected Insect Cells.

Aperçu rapide pour PI3K p85/p55 Protein (Complex) (ABIN7939821)

Antigène: PI3K p85/p55 (Phosphoinositide 3 Kinase, p85/p55 (PI3K p85/p55))

Type de proteíne: Recombinant

Origine: Humain

Source: Baculovirus infected Insect Cells

Application: Activity Assay (AcA), Functional Studies (Func), Screening Assay (ScA)

Détail du produit

Attributs du protein: Complex

Fonction: Recombinant PI3Ka / p85 protein complex

Information d'application

Indications d'application: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Stockage

Format: Liquid

Buffer: Recombinant PI3Ka / p85 Complex is supplied in 25 mM HEPES pH 7.5, 300 mM NaCl, 20 % glycerol, 0.04 % Triton X-100, 0.5 mM TCEP.

Conseil sur la manipulation: Avoid repeated freeze/thaw cycles,keep on ice when not in storage

Stock: -80 °C

Stockage commentaire: Recombinant proteins in solution are temperature sensitive and must be stored at -80°C to prevent degradation. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.

Détail du antigène

Antigène: PI3K p85/p55 (Phosphoinositide 3 Kinase, p85/p55 (PI3K p85/p55))

Autre désignation: PI3Ka & p85

Sujet:

Protein Background: Phosphoinositide 3-kinase (PI3K) family of lipid kinases are divided into three major classes based on primary sequence, substrate preference, and regulation. While class IA PI3Ks are heterodimeric enzymes composed of a catalytic subunit (p110a, p110b, or p110d) complexed with one of five regulatory subunits (p85a, p55a, p50a, p85b, or p55g), the classIB enzyme is a dimer made of p110g catalytic subunit and p101or p84 regulatory subunit. The class I catalytic subunit polypeptides p110a, p110b, p110d, and p110g are encoded by PIK3CA, PIK3CB, PIK3CD, and PIK3CG, respectively. The regulatory subunits are encoded by five genes: PIK3R1 codes p85a, p55a, and p50a, PIK3R2 codes p85b, PIK3R3 codes p55g, PIK3R5 codes p101, and PIK3R6 codes p84. PI3K is a lipid and protein kinase that can be activated by receptor tyrosine kinases, cytokine receptors, chemokine G protein-coupled receptors, or oncogenes, including RAS. PI3K converts phosphatidylinositol 4,5-bisphosphate (PIP2) to phosphatidylinositol-3,4,5-triphosphate (PIP3), which leads to activation of pyruvate dehydrogenase kinase 1 (PDK1) and the serine/threonine kinase AKT (PKB) (7). AKT has many downstream effectors, which regulate the cell cycle, apoptosis, protein translation, autophagy, and metabolism. Most cytokines and RTK ligands signal through class IA PI3K.

Short Description: Recombinant PI3Ka / p85 Complex that includes full length human PI3Ka protein (accession number NP_006209.2) with an N-terminal FLAG tag and full length human p85 protein (accession number NP_852664.1) without tag was expressed in Hi5 cells. The molecular weights of PI3Ka and p85 are 125.6 kDa and 83.6 kDa, respectively.

Aliases: Phosphoinositide 3-kinase, PI3K, P110-Alpha, CLAPO, CLOVE, CCM4, MCAP, MCM