SARS-CoV-2 NSP12 (RdRP) Protéine

N° du produit ABIN7939832

Cette protéine Recombinant SARS-CoV-2 NSP12 (RdRP) est produite dans Baculovirus infected Insect Cells.

Aperçu rapide pour SARS-CoV-2 NSP12 (RdRP) Protéine (ABIN7939832)

Antigène: SARS-CoV-2 NSP12 (RdRP) (SARS-CoV-2 RdRP) (SARS-CoV-2 RNA-dependent RNA Polymerase (NSP12) (SARS-CoV-2 RdRP))

Type de proteíne: Recombinant

Origine: SARS Coronavirus-2 (SARS-CoV-2)

Source: Baculovirus infected Insect Cells

Application: Activity Assay (AcA), Functional Studies (Func), Screening Assay (ScA)

Détail du produit

Fonction: Recombinant SARS-CoV-2 NSP12 / RdRp protein

Information d'application

Indications d'application: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Stockage

Format: Liquid

Buffer: Recombinant SARS-CoV-2 NSP12 / RdRp protein is supplied in 25 mM HEPES-NaOH pH 7.5, 300 mM NaCl, 10 % glycerol, 0.04 % Triton X-100, and 0.5 mM TCEP.

Conseil sur la manipulation: Avoid repeated freeze/thaw cycles,keep on ice when not in storage

Stock: -80 °C

Stockage commentaire: Recombinant proteins in solution are temperature sensitive and must be stored at -80°C to prevent degradation. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.

Détail du antigène

Antigène: SARS-CoV-2 NSP12 (RdRP) (SARS-CoV-2 RdRP) (SARS-CoV-2 RNA-dependent RNA Polymerase (NSP12) (SARS-CoV-2 RdRP))

Autre désignation: SARS-CoV-2 NSP12 & RdRp

Sujet:

Protein Background: Coronavirus replication and transcription machinery involves multiple virus-encoded nonstructural proteins (nsp). Coronaviruses are enveloped positive-stranded RNA viruses with the largest currently known RNA genomes. Expression of their genomes begins with the translation of two large replicase polyproteins, pp1a (>4,000 residues) and pp1ab (>7,000 residues), which are encoded by the viral replicase gene that comprises open reading frame 1a (orf1a) and orf1b1. pp1a and pp1ab are extensively processed by orf1a-encoded proteases to yield 15 or 16 mature nonstructural (replicase) proteins that assemble to form the membrane-associated viral replication and transcription machinery, which is vital to the viral life cycle. The nsp12, which is the first protein encoded by ORF1b, has common viral RNA-dependent RNA polymerase (RdRp) motifs and is thus the catalytic core of the RNA replicase.

Short Description: Recombinant SARS-CoV-2 NSP12 / RdRp protein was expressed in baculovirus expression system as the full length protein (accession number YP_009724389.1) with a N-terminal FLAG tag. The molecular weight of the protein is 107.9 kDa.

Aliases: Nonstructural Protein 12